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MAK433

Sigma-Aldrich

Adenosine Quantification Assay Kit

sufficient for 100 fluorometric tests

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Quality Level

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

relevant disease(s)

cancer; neurological disorders; cardiovascular diseases

storage temp.

−20°C

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Adenosine Quantification Assay Kit sufficient for 100 fluorometric tests

MAK433

Adenosine Quantification Assay Kit

Adenosine suitable for cell culture, BioReagent

A4036

Adenosine

Creatinine Assay Kit sufficient for 100 colorimetric or fluorometric tests

MAK080

Creatinine Assay Kit

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

-

usage

sufficient for 100 fluorometric tests

usage

-

usage

sufficient for 100 colorimetric or fluorometric tests (for choline assay and 50 tests acetylcholine assay)

usage

sufficient for 100 colorimetric or fluorometric tests

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

relevant disease(s)

cancer; neurological disorders; cardiovascular diseases

relevant disease(s)

-

relevant disease(s)

-

relevant disease(s)

genitourinary disorders; neurological disorders

General description

Adenosine is a purine nucleoside base, which is the precursor of adenosine triphosphate, or ATP, and is utilized thoroughly throughout the entire body in general metabolism. Extracellular adenosine acts as a local modulator with a generally cytoprotective function in the body. Its effects on tissue protection and repair fall into four categories: increasing the ratio of oxygen supply to demand; protecting against ischaemic damage by cell conditioning; triggering anti-inflammatory responses; and the promotion of angiogenesis.

Application

The Adenosine Quantification Assay Kit may be used for:
  • Cancer Research
  • Cardiovascular Disease Research
  • Neurological Disease Research

Features and Benefits

  • Supportive calculator (Click here to download a calculator excel file) Analyze results based on your experimental data!
  • Quick instruction bench card - to assure your experimental success
  • More experiments in one kit - contains sufficient reagents for 100 tests

Suitability

The kit is suitable for the quantification of adenosine in plasma.

Principle

The Adenosine Quantification Assay Kit provides a simple and quick fluorometric method for the detection of adenosine. The kit is based on a multi-step enzymatic approach, resulting in the formation of fluorescent product that is measured at λEx = 550 nm/λEm = 585. The kit has a linear range of 0 to 20 μM.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


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Adenosine receptors as therapeutic targets.
Jacobson K A, et al.
Nature Reviews. Drug Discovery, 5(3), 247-264 (2006)
B B Fredholm et al.
Pharmacological reviews, 53(4), 527-552 (2001-12-06)
Four adenosine receptors have been cloned and characterized from several mammalian species. The receptors are named adenosine A(1), A(2A), A(2B), and A(3). The A(2A) and A(2B) receptors preferably interact with members of the G(s) family of G proteins and the
Monika Samsel et al.
Postepy higieny i medycyny doswiadczalnej (Online), 67, 1189-1203 (2014-01-01)
Adenosine is an endogenous purine nucleoside that plays an important role in many biochemical processes. It acts through the four types of adenosine receptors: A1, A2A, A2B, A3 which are located i.a. in the immune, nervous, circulatory, respiratory or urinary

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