Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

MAK525

Sigma-Aldrich

Acid Phosphatase Fluorometric Assay Kit

sufficient for 100 fluorometric tests

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Quality Level

detection method

fluorometric

relevant disease(s)

genitourinary disorders; cancer; gastrointestinal diseases

shipped in

wet ice

storage temp.

−20°C

General description

Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used.

Features and Benefits

  • Linear detection range: 0.008 to 10 U/L .
  • Compatible with high-throughput handling systems.

Suitability

The kit is suitable for acid phosphatase activity determination in biological samples (e.g. plasma, serum, cell lysate, tissue samples).

Principle

The Acid Phosphatase Fluorometric Assay Kit is based on the cleavage of a synthetic substrate. The product methylumbelliferone (MUB) becomes intensely fluorescent after addition of the stop reagent. The increase in fluorescence at λEx = 360 nm/λEm = 450 nm after addition of the stop reagent is directly proportional to the enzyme activity.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service