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MREH01

Sigma-Aldrich

MISSION® Target ID Library

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NACRES:
NA.51

packaging

pkg of 100 μL (library)
pkg of 200 μL (primer 1)
pkg of 200 μL (primer 2)

Quality Level

concentration

1 ng/μL (library)
25 μM (primer 1)
25 μM (primer 2)

shipped in

wet ice

storage temp.

−20°C

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shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

packaging

pkg of 100 μL (library)

packaging

-

packaging

-

packaging

-

General description

The Target ID Library enables bench-top transcriptome-wide human miRNA and ncRNA gene target identification. It has an innovative dual selection system, which makes rapid whole transcriptome miRNA and ncRNA gene target screens accessible to any researcher with minimal reagent, time, or capital equipment expense.

The Target ID Library is a transcriptome wide cDNA pool produced using a proprietary process. Each library is tested for gene representation to ensure robust library coverage. The library is provided in ready-to-use DNA format, with enough material for multiple genome-wide screens.

The Target ID Library is a pool of plasmids, each with a human cDNA inserted into the 3′-UTR region of a thymidine kinase-zeocin fusion protein (Tkzeo). Dual-selection allows the user to transfect cells and screen the entire library at once, selecting first for stable transformants and secondly, after introducing a miRNA or ncRNA of interest, for target cDNAs. Selected cDNAs are identified by sequencing

Download deep sequencing data of target ID library.

Components

Library: 100μl (approx 1μg/μl), frozen liquid, store at -10°C to -25°C.
Primer 1: 200μl (25μM), frozen liquid, store at -10°C to -25°C
Primer 2: 200μl (25μM), frozen liquid, store at -10°C to -25°C

Legal Information

MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

required but not provided

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Joop Gäken et al.
Nucleic acids research, 40(10), e75-e75 (2012-02-11)
MicroRNAs (miRNA) are a class of small RNA molecules that regulate numerous critical cellular processes and bind to partially complementary sequences resulting in down-regulation of their target genes. Due to the incomplete homology of the miRNA to its target site
Shok Ping Lim et al.
Journal of leukocyte biology, 103(5), 855-866 (2018-04-16)
MicroRNAs are short endogenous noncoding RNAs that play pivotal roles in a diverse range of cellular processes. The miR-181 family is important in T cell development, proliferation, and activation. In this study, we have identified BRK1 as a potential target

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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