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MTOX1300

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Canine MDCKII Wild Type Cells

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Synonym(s):
MDCK II, MDCK-2, MDCKII-WT
NACRES:
NA.81

biological source

canine

form

liquid

storage temp.

−196°C

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This Item
MTOX130312122002553325
vibrant-m

MTOX1300

Canine MDCKII Wild Type Cells

vibrant-m

12122002

REM 134 Cell Line canine

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−70°C

form

liquid

form

liquid

form

-

form

liquid

General description

MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435).

Application

Canine MDCKII Wild Type Cells can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells.
The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells. See technical bulletin for detailed protocols.

Features and Benefits

MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron.

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Storage and Stability

Upon receiving a shipment of frozen canine MDCKII wild type cells it is important the end user gives the shipment attention without delay. To ensure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at –70 °C. Storage at –70 °C will result in loss of viability.

Legal Information

Exhibit 1: ADME/Tox cell lines licenseMDCKII subclone was originally isolated by Daniel Louvard, Institut Curie, Paris France.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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D Louvard
Proceedings of the National Academy of Sciences of the United States of America, 77(7), 4132-4136 (1980-07-01)
A dog kidney epithelial cell line (MDCK), grown in monolayer, displayed in vitro an asymmetric localization of surface proteins. Aminopeptidase [alpha-aminoacylpeptide hydrolase (microsomal), EC 3.4.11.2] was found only in the apical face whereas Na+, K+-ATPase (ATP phosphohydrolase, EC 3.6.1.3) was
G C Hansson et al.
The EMBO journal, 5(3), 483-489 (1986-03-01)
The glycosphingolipids (GSLs) of two sublines of Madin-Darby canine kidney (MDCK) cells, an epithelial cell line, were characterized by t.l.c., antibody overlay and mass spectrometry. The major characteristic which distinguishes the two MDCK cell strains is their trans-epithelial electrical resistance

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