biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
NOS-3F7-B11-B5, monoclonal
species reactivity
mouse, bovine, rat
technique(s)
immunohistochemistry (frozen sections): 1:100
western blot: 1:50-1:500
isotype
IgM
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Nos1(18125)
rat ... Nos1(24598)
Specificity
The antibody reacts with iNOS, eNOS and nNOS from rat and bovine, and with iNOS in induced RAW 264.7 mouse macrophages. In immunoblotting, the antibody detects a band at 130 kDa representing iNOS in samples first induced with IFN (interferon) and LPS (lipopolysaccharides). It also detects a band at 155 kDa in tissues expressing nNOS and a band at 140 kDa in tissues expressing eNOS. The antibody may be used to localize and detect the three isoforms of nitric oxide synthase (NOS): neuronal or brain (nNOS or bNOS), endothelial (eNOS) and inducible (iNOS).
Immunogen
bovine cerebellum NOS.
Biochem/physiol Actions
Neuronal nitric acid synthase (Nos1) in involved in the production of nitric oxide. Mutations in the gene encoding it increases the susceptibility to behavioral dysfunction after an exposure to alcohol in mice.
Physical form
Ascites fluid containing ≤0.1% sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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