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N218

Sigma-Aldrich

Monoclonal Anti-Nitric Oxide Synthase, Universal antibody produced in mouse

clone NOS-3F7-B11-B5, ascites fluid

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Synonym(s):
Anti-IHPS1, Anti-N-NOS, Anti-NC-NOS, Anti-NOS, Anti-bNOS, Anti-nNOS
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

NOS-3F7-B11-B5, monoclonal

species reactivity

mouse, bovine, rat

technique(s)

immunohistochemistry (frozen sections): 1:100
western blot: 1:50-1:500

isotype

IgM

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Nos1(18125)
rat ... Nos1(24598)

Specificity

The antibody reacts with iNOS, eNOS and nNOS from rat and bovine, and with iNOS in induced RAW 264.7 mouse macrophages. In immunoblotting, the antibody detects a band at 130 kDa representing iNOS in samples first induced with IFN (interferon) and LPS (lipopolysaccharides). It also detects a band at 155 kDa in tissues expressing nNOS and a band at 140 kDa in tissues expressing eNOS. The antibody may be used to localize and detect the three isoforms of nitric oxide synthase (NOS): neuronal or brain (nNOS or bNOS), endothelial (eNOS) and inducible (iNOS).

Immunogen

bovine cerebellum NOS.

Biochem/physiol Actions

Neuronal nitric acid synthase (Nos1) in involved in the production of nitric oxide. Mutations in the gene encoding it increases the susceptibility to behavioral dysfunction after an exposure to alcohol in mice.

Physical form

Ascites fluid containing ≤0.1% sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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