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N2280

Sigma-Aldrich

Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody produced in mouse

clone NOS-B1, ascites fluid

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Synonym(s):
Anti-bNOS
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

NOS-B1, monoclonal

mol wt

antigen 150-160 kDa

contains

15 mM sodium azide

species reactivity

rat, goat, pig, human

technique(s)

microarray: suitable
western blot: 1:3,000 using fresh rat cerebellum extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NOS1(4842)
rat ... Nos1(24598)

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This Item
N7280N7155C9672
species reactivity

rat, goat, pig, human

species reactivity

rat

species reactivity

human, rat

species reactivity

rat, human

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

mouse

Gene Information

human ... NOS1(4842)
rat ... Nos1(24598)

Gene Information

human ... NOS1(4842)
rat ... Nos1(24598)

Gene Information

human ... NOS1(4842)
rat ... Nos1(24598)

Gene Information

human ... NCAM1(4684), NCAM2(4685)
rat ... Ncam1(24586), Ncam2(288280)

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

clone

NOS-B1, monoclonal

clone

polyclonal

clone

polyclonal

clone

NCAM-0B11, monoclonal

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General description

Monoclonal Anti-Nitric Oxide Synthase-Brain (bNOS) (mouse IgG1 isotype) is derived from the NOS-B1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Nitric oxide synthase (NOS) has been localized in many different cell types. Type I NOS is found in neurons. It is a 150-160 kDa protein, also called NOS-1, neuronal NOS (nNOS), brain NOS (bNOS), cerebral NOS, constitutive NOS or Ca2+- regulated NOS (cNOS). bNOS is present also in skeletal muscle, where it is complexed with dystrophin, and is absent in Duchenne′s muscular dystrophy, which perhaps accounts for symptoms of the disease.

Specificity

Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody is specific for nitric oxide synthase (NOS) derived from brain (bNOS, 150-160 kDa and several breakdown products of lower M.W.). The product does not bind to NOS derived from macrophages (mNOS) and endothelial cells (eNOS). This antibody is specific for bNOS in humans, goats, pigs and rats.

Immunogen

recombinant neuronal NOS fragment (amino acids 1-181) from rat brain.

Application

Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody produced in mouse has been used in:
  • western blot analysis
  • immunocytochemistry
  • double immunofluorescence
  • immunofluorescence staining
  • enzyme-linked immunosorbent assay (ELISA)
  • dot blot immunoassay
  • immunohistochemical staining

Biochem/physiol Actions

Nitric oxide synthase is an enzyme that catalyzes the formation of nitric oxide. Nitric oxide is responsible for regulating several biochemical functions such as hemostasis, neurotransmission and vascular injury response . Mutations in nitric oxide synthase gene have been associated with diabetic nephropathy.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Ben Coomber et al.
Frontiers in neurology, 6, 53-53 (2015-03-26)
A significant challenge in tinnitus research lies in explaining how acoustic insult leads to tinnitus in some individuals, but not others. One possibility is genetic variability in the expression and function of neuromodulators - components of neural signaling that alter
Chadd M Funk et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(38), 9132-9148 (2017-08-20)
During non-rapid eye-movement (NREM) sleep, cortical and thalamic neurons oscillate every second or so between ON periods, characterized by membrane depolarization and wake-like tonic firing, and OFF periods, characterized by membrane hyperpolarization and neuronal silence. Cortical slow waves, the hallmark
The distribution of nitric oxide synthase in the inferior colliculus of guinea pig
Coote EJ, et al.
Neuroscience, 154(1), 218-225 (2008)
Elevated expression of CAPON and neuronal nitric oxide synthase in the sciatic nerve of rats following constriction injury
Cui Z, et al.
The Veterinary Journal, 187(3), 374-380 (2011)
R C Rogers et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 285(2), R479-R489 (2003-04-26)
Activation of esophageal mechanosensors excites neurons in and near the central nucleus of the solitary tract (NSTc). In turn, NSTc neurons coordinate the relaxation of the stomach [i.e., the receptive relaxation reflex (RRR)] by modulating the output of vagal efferent

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