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N3910

Sigma-Aldrich

Neutralization Solution B

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Synonym(s):
DNA neutralization solution, neutralization buffer DNA extraction

grade

for molecular biology

Quality Level

form

liquid

technique(s)

DNA amplification: suitable
DNA extraction: suitable

storage temp.

−20°C

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This Item
N9784D5688T9285
vibrant-m

N3910

Neutralization Solution B

vibrant-m

N9784

Neutralization Solution for Blood

vibrant-m

D5688

Dilution Solution

vibrant-m

T9285

Tris-EDTA buffer solution

grade

for molecular biology

grade

for molecular biology

grade

-

grade

for molecular biology

technique(s)

DNA amplification: suitable, DNA extraction: suitable

technique(s)

-

technique(s)

-

technique(s)

-

storage temp.

−20°C

storage temp.

room temp

storage temp.

−20°C

storage temp.

room temp

form

liquid

form

liquid

form

liquid

form

liquid

General description

This Neutralization Buffer B is intended for use with Sigma′s REDExtract-N-Amp Tissue PCR Kits. This buffer is used to neutralize the DNA extract and be combined with the REDExtract-N-Amp PCR Reaction Mix and PCR primers to amplify target DNA. The REDExtract-N-Amp Kits include a sufficient volume of Neutralization Buffer B for at least one neutralization per extraction. However, where additional amplifications are required, supplemental Neutralization buffer may be needed.

Application

Neutralization Solution B has been used in the modified Sigma Extract-N-Amp extraction procedure. It has also been used in genotyping of mice, for neutralizing the DNA extract prior to polymerase chain reaction (PCR) using Sigma′s REDExtract-N-Amp PCR Reaction Mix.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

pictograms

Exclamation markEnvironment

signalword

Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Protocols

The SYBR® Green Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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