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N7280

Sigma-Aldrich

Anti-Nitric Oxide Synthase, Brain (1409-1429) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Synonym(s):
Anti-bNOS
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 150-160 kDa

species reactivity

rat

technique(s)

western blot: 1:10,000 using rat brain extract

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NOS1(4842)
rat ... Nos1(24598)

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N7155N2280N3893
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

ascites fluid

antibody form

IgG fraction of antiserum

technique(s)

western blot: 1:10,000 using rat brain extract

technique(s)

microarray: suitable, western blot: 1:10,000 using rat brain extract

technique(s)

microarray: suitable, western blot: 1:3,000 using fresh rat cerebellum extract

technique(s)

immunohistochemistry (frozen sections): 1:100 using acetone-fixed, frozen tissue sections of mouse heart, microarray: suitable, western blot: 1:10,000 using bovine lung endothelial cell extract

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

buffered aqueous solution

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General description

In humans, nitric oxide synthase 1 (NOS1) or neuronal nitric acid synthase (nNOS) is localized to the sarcoplasmic reticulum. The gene encoding it is, localized on human chromosome 12q24.22. In rats, the protein is present in perivascular nerves supplying the cerebral vasculature. The gene encoding it is localized on rat chromosome 12q16.
The nitric oxide synthase (NOS) isoform found in neurons is a 150-160 kDa protein. It is also termed brain NOS, NOS1, neuronal NOS (nNOS or bNOS, neuronal constitutive NOS or Ca2+-regulated NOS (cNOS, ncNOS). The nNOS or NOS1 gene is mapped to human chromosome 12q24.22. bNOS is present in skeletal muscle, central, peripheral neurons and neuronal cell bodies. Human bNOS and endothelial nitric oxide synthase (eNOS) share 52% amino acid identity, and rat and human bNOS share 93% amino acid identity. Structurally NOS exists as homodimers. The N-terminal region comprises heme and calmodulin binding sites. The reduced nicotinamide adenine dinucleotide phosphate (NADPH) and flavins binding regions are localized in C-terminal region.

Specificity

The antibody is specific for nitric oxide synthase (NOS) derived from brain. It does not recognize NOS derived from activated macrophages (iNOS) and endothelial cells (eNOS).

Immunogen

synthetic peptide corresponding to nitric oxide synthase (NOS) of rat brain origin (bNOS, amino acids 1409-1429) conjugated to KLH. The immunogen sequence differs from the human bNOS by a single amino acid residue.

Application

Anti-Nitric Oxide Synthase, Brain (1409-1429) antibody produced in rabbit has been used in:
  • immunoblotting
  • immunostaining
  • immunohistochemistry
  • immunofluorescence
  • immunocytochemistry

Biochem/physiol Actions

Nitric oxide synthase (NOS), brain (nNOS or bNOS) mediates long-term regulation of synaptic transmission (long-term potentiation, long-term inhibition). It catalyzes the production of nitric oxide (NO) from L-arginine. The NO mediates neurovascular coupling and is associated with neuronal plasticity and pain signal transmission. nNOS regulates blood pressure, relaxation of smooth muscle and vasodilatation via peripheral nitrergic nerves. nNOS is complexed to dystrophin. However, in Duchenne muscular dystrophy (DMD), the skeletal muscle sarcolemma is devoid of nNOS.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Storage and Stability

Store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Kinga Gzielo et al.
Cellular and molecular neurobiology, 37(5), 783-789 (2016-08-20)
Obesity in humans is associated with cognitive decline and elevated risk of neurodegenerative diseases of old age. Variations of high-fat diet are often used to model these effects in animal studies. However, we previously reported improvements in markers of memory
Michael Leist et al.
Scientific reports, 6, 24904-24904 (2016-04-29)
Although hyperpolarization-activated cyclic nucleotide-gated cation (HCN) channels and the corresponding h-current (Ih) have been shown to fundamentally shape the activity pattern in the thalamocortical network, little is known about their function in local circuit GABAergic interneurons (IN) of the dorsal
Human Ischemic Cardiomyopathy Shows Cardiac Nos1 Translocation and its Increased Levels are Related to Left Ventricular Performance
Esther R
Scientific Reports (2016)
Gene-based anchoring of the rat genetic linkage and cytogenetic maps: new regional localizations, orientation of the linkage groups, and insights into mammalian chromosome evolution
Mammalian Genome (1998)
Depolarization of mitochondria in neurons promotes activation of nitric oxide synthase and generation of nitric oxide
Prasad V
American Journal of Physiology. Heart and Circulatory Physiology (2016)

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