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P0089

Sigma-Aldrich

Anti-Prox1 antibody , Mouse monoclonal

clone 4G10, purified from hybridoma cell culture

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Synonym(s):
Anti-Homeobox prospero-like protein Prox1, Anti-Prospero homeobox 1
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

4G10, monoclonal

form

buffered aqueous solution

mol wt

antigen ~84 kDa

species reactivity

chicken, rat, lizard, human

concentration

~1.5 mg/mL

technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HepG2 cells
western blot: suitable

isotype

IgG2b

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PROX1(5629)
rat ... Prox1(305066)

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MAB5654SAB1306485MAB5652
vibrant-m

MAB5654

Anti-Prox1 Antibody, clone 4G10

vibrant-m

MAB5652

Anti-Prox1 Antibody, clone 5G10

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

mouse

species reactivity

chicken, rat, lizard, human

species reactivity

chicken, mouse, rat, human, lizard

species reactivity

human, mouse

species reactivity

human, mouse, chicken, lizard, rat

antibody form

purified from hybridoma cell culture

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

purified antibody

conjugate

unconjugated

conjugate

-

conjugate

-

conjugate

-

clone

4G10, monoclonal

clone

4G10, monoclonal

clone

polyclonal

clone

5G10, monoclonal

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General description

Monoclonal Anti-Prox1 (mouse IgG2b isotype) is derived from the hybridoma 4G10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant fusion protein of human Prox1. Prospero related homeobox gene 1 (Prox1) belongs to transcription factor subfamily and is homologous to Drosophila homeobox protein, prospero.

Immunogen

recombinant fusion protein corresponding to amino acids 547-737 of human Prox1.

Application

Anti-Prox1 antibody, Mouse monoclonal has been used in various immunochemical techniques.

Biochem/physiol Actions

Prospero related homeobox gene 1 (Prox1) is a key regulator for neuroblast fate determination and photoreceptor development. Prox1 regulates cell differentiation and organogenesis in lens, retina, lymphatic vessels, liver and pancreas. It was found to act as a tumor suppressor gene, because its deficiency or its mutated forms have been implicated in various human cancers. Knockdown of Prox1 by siRNA or by RNA mutations significantly accelerated the in vitro and in vivo growth of hepatocellular carcinoma (HCC) as well as esophageal cancer cells. Prox1 is hypermethylated and transcriptionally silenced in primary and metastatic breast cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Loss of Function of the Candidate Tumor Suppressor prox1 by RNA Mutation in Human Cancer Cells
Takahashi M, et al.
Neoplasia, 8(12), 1003-1010 (2006)
Epigenetic silencing of the candidate tumor suppressor gene PROX1 in sporadic breast cancer
Versmold B, et al.
International Journal of Cancer. Journal International Du Cancer, 121(3), 547-554 (2007)
Xiaoren Chen et al.
Hybridoma (2005), 25(1), 27-33 (2006-02-16)
Prox1 is a divergent homeodomain protein important for the development of the lens, retina, liver, pancreas, and lymphatic vasculature. Prox1 expression is highly upregulated in transformed hepatocytes and has been used as a marker to distinguish lymphatic from blood vasculature.
Molecular mechanisms of lymphatic vascular development
Makinen T, et al.
Cellular and Molecular Life Sciences, 64(15), 1915-1929 (2007)
Guadalupe Álvarez-Hernán et al.
Experimental eye research, 209, 108681-108681 (2021-06-25)
Planar cell polarity (PCP) is evolutionary conserved and play a critical role in proper tissue development and function. During central nervous system development, PCP proteins exhibit specific patterns of distribution and are indispensable for axonal growth, dendritogenesis, neuronal migration, and

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