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P0372

Sigma-Aldrich

Anti-Podocin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Podocin Antibody, Podocin Antibody - Anti-Podocin antibody produced in rabbit
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~42 kDa (doublet)

species reactivity

human, rat, mouse

technique(s)

indirect immunofluorescence: 10-20 μg/mL using acetone-fixed human or rat kidney frozen sections
western blot (chemiluminescent): 0.5-1 μg/mL using whole extract of rat glomeruli

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NPHS2(7827)
mouse ... Nphs2(170484)
rat ... Nphs2(170672)

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This Item
P1995SAB4200810SAB1406635
vibrant-m

SAB4200810

Anti-Podocin, Mouse monoclonal

Quality Level

200

Quality Level

200

Quality Level

-

Quality Level

100

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

mouse

technique(s)

indirect immunofluorescence: 10-20 μg/mL using acetone-fixed human or rat kidney frozen sections, western blot (chemiluminescent): 0.5-1 μg/mL using whole extract of rat glomeruli

technique(s)

indirect immunofluorescence: 10-20 μg/mL using rat kidney frozen sections, western blot (chemiluminescent): 2.5-5 μg/mL using whole extract of rat kidney glomeruli and rat lung

technique(s)

immunoblotting: 0.25-0.5 μg/mL using using human HEK-293T cells., immunofluorescence: 1-2 μg/mL using human HEK-293T cells.

technique(s)

western blot: 1 μg/mL

shipped in

dry ice

shipped in

dry ice

shipped in

-

shipped in

dry ice

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General description

Podocin is a podocyte structural protein, that belongs to the band-7-stomatin family of lipid raft-associated proteins. This family contains more than 1800 conserved proteins which share ∼150 amino acid domains showing similarity to mitochondrial protein prohibitin (PHB).
Podocin is a hairpin-like integral membrane protein with intracellular N- and C- termini. Podocin is located at the insertion site of the slit membrane, an intercellular junction found in mammalian kidney.

Specificity

Anti-Podocin recognizes human, mouse, and rat podocin.

Immunogen

Synthetic peptide corresponding to amino acid residues of human podocin with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse and differs by one amino acid in rat.

Application

Anti-Podocin antibody produced in rabbit has been used for Western Blotting and immunohistochemistry.

Biochem/physiol Actions

Podocin and nephrin are essential for maintaining the slit diaphragm. Studies show that mutations in this gene account for around 15% cases of nephrotic syndrome cases. Podocin interacts with cholesterol and promotes the formation of protein-lipid supercomplexes, which control ion channel complexes. The podocin gene is known to be associated with autosomal recessive SRNS (nephrotic syndrome type 2). Podocin actively participates in the ultrafiltration process occuring in kidney.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Customers Also Viewed

Slide 1 of 3

1 of 3

Excess podocyte semaphorin-3A leads to glomerular disease involving plexinA1-nephrin interaction.
Reidy KJ et al
The American Journal of Pathology, 183(4), 1156-1168 (2013)
Semaphorin3a promotes advanced diabetic nephropathy.
Aggarwal PK et al
Diabetes, 64(5), 1743-1759 (2015)
NPHS2 mutations account for only 15% of nephrotic syndrome cases.
Guaragna MS et al
BMC Medical Genetics, 16(88) (2015)
Batoul Wehbi et al.
Journal of the American Society of Nephrology : JASN, 30(7), 1238-1249 (2019-06-23)
IgA nephropathy (IgAN) often follows infections and features IgA mesangial deposition. Polymeric IgA deposits in the mesangium seem to have varied pathogenic potential, but understanding their pathogenicity remains a challenge. Most mesangial IgA1 in human IgAN has a hypogalactosylated hinge
Induction of podocyte VEGF164 overexpression at different stages of development causes congenital nephrosis or steroid-resistant nephrotic syndrome.
Veron D et al
The American Journal of Pathology, 177(5), 2225-2233 (2010)

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