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P1782

Sigma-Aldrich

Protein kinase Cα isozyme human

≥70% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous glycerol solution

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Synonym(s):
PKCα
MDL number:
NACRES:
NA.32

recombinant

expressed in baculovirus infected insect cells

Quality Level

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

≥250 units/μg protein

mol wt

80-81 kDa by SDS-PAGE

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRKCA(5578)

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Sigma-Aldrich

P1782

Protein kinase Cα isozyme human

Sigma-Aldrich

P8538

Protein Kinase Cδ isozyme human

Sigma-Aldrich

P0194

Protein Kinase Cζ isozyme human

Sigma-Aldrich

K4643

PKCθ, active, GST tagged human

Gene Information

human ... PRKCA(5578)

Gene Information

human ... PRKCD(5580)

Gene Information

human ... PRKCZ(5590)

Gene Information

human ... PRKCQ(5588)

assay

≥70% (SDS-PAGE)

assay

>95% (SDS-PAGE)

assay

≥75% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

recombinant

expressed in baculovirus infected insect cells

recombinant

expressed in baculovirus infected insect cells

recombinant

expressed in baculovirus infected insect cells

recombinant

expressed in baculovirus infected Sf9 cells

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

buffered aqueous glycerol solution

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

Biochem/physiol Actions

Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatidylinositol diphosphate (PIP2) and phosphatidylcholine (PC) through the action of various activated phospholipases. Phorbol esters also stimulate PKC. At least 11 PKC isozymes have been identified that differ in primary structure, tissue distribution, subcellular localization, response to extracellular signals, and substrate specificity. The isozymes can be grouped into three subfamilies. Members of the first family require Ca2+ and phospholipid and include PKCα, βI, βII, and γ. Members of the second family are phospholipid-dependent but Ca2+-independent, and include PKCδ, ε, η, and θ. Members of the third family are not activated by either DAG or phorbol esters and include PKCξ, μ, and ι.
Phosphorylation appears to be an important mechanism of regulation of all PKCs. PKC plays a role in the regulation of cell transformation, growth, differentiation, ruffling, vesicle trafficking, apoptosis and gene expression.

Unit Definition

One unit will transfer 1 pmol of phosphate from ATP to Histone H1 in 1 minute at pH 7.4 at 30 °C

Physical form

Supplied as a buffered aqueous glycerol solution, containing Trizma buffer, NaCl, DTT and Glutathione.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Heidi Budde et al.
Antioxidants (Basel, Switzerland), 10(7) (2021-08-07)
Oxidative stress is defined as an imbalance between the antioxidant defense system and the production of reactive oxygen species (ROS). At low levels, ROS are involved in the regulation of redox signaling for cell protection. However, upon chronical increase in

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