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P3644

Sigma-Aldrich

L-α-Phosphatidylcholine

from soybean, Type IV-S, ≥30% (enzymatic)

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Synonym(s):
1,2-Diacyl-sn-glycero-3-phosphocholine, 3-sn-Phosphatidylcholine, L-α-Lecithin, Azolectin, PC
CAS Number:
Beilstein/REAXYS Number:
5209585
EC Number:
NACRES:
NA.25

biological source

soybean

Quality Level

type

Type IV-S

form

solid

concentration

≥30% (enzymatic)

solubility

chloroform: soluble 100 mg/mL, clear to slightly hazy, yellow to orange

functional group

phospholipid

lipid type

phosphoglycerides

shipped in

ambient

storage temp.

−20°C

InChI

1S/C42H80NO8P/c1-6-8-10-12-14-16-18-20-21-23-25-27-29-31-33-35-42(45)51-40(39-50-52(46,47)49-37-36-43(3,4)5)38-48-41(44)34-32-30-28-26-24-22-19-17-15-13-11-9-7-2/h14,16,20-21,40H,6-13,15,17-19,22-39H2,1-5H3/b16-14-,21-20-/t40-/m1/s1

InChI key

JLPULHDHAOZNQI-ZTIMHPMXSA-N

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This Item
P5638P7443P5394
L-α-Phosphatidylcholine from soybean, Type IV-S, ≥30% (enzymatic)

P3644

L-α-Phosphatidylcholine

L-α-Phosphatidylcholine from soybean, Type II-S, 14-29% choline basis

P5638

L-α-Phosphatidylcholine

L-α-Phosphatidylcholine from soybean, ≥99% (TLC), lyophilized powder

P7443

L-α-Phosphatidylcholine

L-α-Phosphatidylcholine from dried egg yolk, Type X-E, ≥40% (enzymatic)

P5394

L-α-Phosphatidylcholine

functional group

phospholipid

functional group

phospholipid

functional group

phospholipid

functional group

phospholipid

form

solid

form

powder or solid

form

lyophilized powder

form

solid

solubility

chloroform: soluble 100 mg/mL, clear to slightly hazy, yellow to orange

solubility

-

solubility

-

solubility

-

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Application

L-α-Phosphatidylcholine is suitable for use:
  • as a component of human erythroid massive amplification culture (HEMAdef) medium for clinical expansion of human erythroblasts
  • in the chloride efflux assay to examine the chloride permeability of lipid vesicles conferred by soluble CLIC1
  • in an assay to measure solubilized wax synthase activity
  • as a component of buffer A used for the resuspension of the cholate-solubilized catalytic unit of adenylate cyclase
  • as a cryoprotectant for freezing ruminant sperm
  • as a substrate for the estimation of phospholipase activity
  • to make liposomes to assess their efficiency of recruitment of ARF (ADP ribosylation factor) and AP-1 (adaptor protein)
  • in the preparation of the isolated catalytic unit of soluble adenylate cyclase

Biochem/physiol Actions

Phosphatidylcholine is the major membrane phospholipid in eukaryotic cells, forming the structural component of these membranes. It also serves as a reservoir for several lipid messengers and a source for several bioactive lipids such as, lysophosphatidylcholine, phosphatidic acid, diacylglycerol, lysophosphatidylcholine, platelet activating factor, and arachidonic acid.
A major structural phospholipid in brain, comprising approx. 15% of total lipid; primarily localized to gray matter.

Preparation Note

Purified from P 5638

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Anna Kloda et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(5), 1540-1545 (2007-01-24)
In this study, the heteromeric N-methyl-D-aspartate (NMDA) receptor channels composed of NR1a and NR2A subunits were expressed, purified, reconstituted into liposomes, and characterized by using the patch clamp technique. The protein exhibited the expected electrophysiological profile of activation by glutamate
R S Salter et al.
The Journal of biological chemistry, 256(19), 9830-9833 (1981-10-10)
The catalytic and guanine nucleotide regulatory (G/F) units of solubilized bovine brain adenylate cyclase were separated by gel filtration as described by Strittmatter, S., and Neer, E. J. ((1980) Proc. Natl. Acad. Sci. U. S. A. 77, 6344-6348). The isolated
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Chiu S C and Tzean S S.
Physiological & Molecular Plant Pathology, 46, 83-94 (1995)
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The aim of this study was to evaluate the effects of glycerol, ethylene glycol or DMSO in a soybean lecithin extender for freezing ram semen. In this study, 20 ejaculates were collected from four Ghezel rams and diluted with soybean

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