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P5498

Sigma-Aldrich

Anti-Pro-NGF antibody produced in rabbit

affinity isolated antibody, lyophilized powder

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Synonym(s):
Anti-Pro-Nerve Growth Factor
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

mouse, rat, human

technique(s)

immunohistochemistry: suitable
western blot: 1:200-1:500

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NGFR(4804)
mouse ... Ngfr(18053)
rat ... Ngfr(24596)

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This Item
N3908SAB1306228HPA004765
conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

technique(s)

immunohistochemistry: suitable, western blot: 1:200-1:500

technique(s)

immunohistochemistry (frozen sections): 1:10,000 using rat brain, indirect immunofluorescence: 1:500 using NGF-treated PC12 cells, microarray: suitable, western blot: 1:5,000 using whole cell extract of NGF-treated PC12 cells

technique(s)

immunohistochemistry: 1:10-1:50, western blot: 1:1000

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:500-1:1000

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Specificity

Does not cross react with pro-BDNF, pro-NT-3, or mature NGF.

Immunogen

peptide corresponding to amino acid residues 84-104 of rat NGF (precursor). The sequence is identical in mouse and 16/21 residues identical in human.

Physical form

Lyophilized from phosphate buffered saline, pH 7.4, 1% BSA and <0.1% sodium azide

Preparation Note

Reconstitute the lyophilized vial with 0.2 ml deionized water.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Warning

Hazard Classifications

Acute Tox. 4 Dermal - Aquatic Chronic 3

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Fan Zhang et al.
Molecular neurobiology, 58(9), 4237-4250 (2021-05-10)
Maternal subclinical hypothyroidism (SCH) during pregnancy can adversely affect the neurodevelopment of the offspring. The balance of nerve growth factor (NGF)-related tropomyosin receptor kinase A/p75 neurotrophin receptor (TrkA/p75NTR) signaling in the hippocampus is important in brain development, and whether it
Elahe Karimi et al.
Drug design, development and therapy, 13, 2513-2532 (2019-08-24)
Nowadays, the drug delivery system is important in the treatment of diseases. A polymeric nanoparticle modified by oleic acid (NPMO) as a Thymol (Thy) drug release system was synthesized from Thymbra spicata and its neurotrophic and angiogenic effects on rat's
Sabah Shiri et al.
RSC advances, 9(67), 38912-38927 (2019-11-27)
Drug delivery systems have been of interest to researchers. The effects of synthesized nano-polymers as silibinin and silymarin extract drug delivery systems on olfactory ensheathing cells under normal and high-glucose conditions were studied. The structure of the nanopolymer was characterized
Li Duan et al.
PloS one, 8(9), e73768-e73768 (2013-09-17)
While aberrant activation of microglial cells was evidently involved in neuroinflammation and neurotoxicity in the neurodegenerative diseases such as Alzheimer's and Parkinson's disease, objective of study was to address if activated microglias deliver their effect by releasing pro-neurotrophins. By in
Yi Xia et al.
International journal of molecular sciences, 14(7), 14085-14104 (2013-07-25)
Growing evidence has shown that proNGF-p75NTR-sortilin signaling might be a crucial factor in neurodegeneration, but it remains unclear if it may function in nigral neurons under aging and disease. The purpose of this study is to examine and quantify proNGF

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