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P7119

Sigma-Aldrich

PGO Enzyme Preparation

1 G capsules

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Synonym(s):
Peroxidase - Glucose oxidase preparation
NACRES:
NA.54

Quality Level

form

powder

solubility

H2O: soluble capsule/100 mL at 25 °C, clear, colorless (pH 6.95-7.05)

storage temp.

2-8°C

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Sigma-Aldrich

P7119

PGO Enzyme Preparation

Peroxidase from horseradish Type I, essentially salt-free, lyophilized powder, ≥50 units/mg solid (using pyrogallol)

P8125

Peroxidase from horseradish

Peroxidase from horseradish Type VI, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol)

P8375

Peroxidase from horseradish

Peroxidase from horseradish Type XII, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol)

P8415

Peroxidase from horseradish

solubility

H2O: soluble capsule/100 mL at 25 °C, clear, colorless (pH 6.95-7.05)

solubility

0.1 M phosphate buffer: soluble (pH 6.0), H2O: soluble

solubility

0.1 M phosphate buffer: soluble 10 mg/mL, clear, orange to red (pH 6.0)

solubility

0.1 M phosphate buffer: soluble (pH 6.0), H2O: soluble

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Each capsule contains 500 units of glucose oxidase (Aspergilus niger), 100 purpurogallin units of peroxidase (horseradish), and buffer salts. The reaction produces oxidized o-Dianisidine, which is brown. The intensity of the brown color measured at 425-475 nm is proportional to the original glucose concentration.

Application

PGO Enzymes preparation has been used:
  • in diet sampling and chemical analyses
  • to determine plasma and follicular fluid glucose levels
  • to measure glucose content in fractions of retrograded debranched rice starch

Biochem/physiol Actions

PGO Enzymes are used for the quantitative, enzymatic determination of glucose in aqueous solutions such as serum. The reactions are normally monitored at 425-475 nm utilizing o-dianisidine as a colorimetric substrate. Glucose oxidase methods eliminate the effects of interfering substances. Contents of one capsule includes 100 units peroxidase and 500 units glucose oxidase and buffer salts.

Suitability

Tested and found suitable for use in the quantitation of glucose.

Preparation Note

The PGO enzymes solution is prepared by adding the contents of 1 capsule of PGO enzymes to 100 mL of water in an amber bottle. Bottle should be inverted several times with gentle shaking to dissolve. PGO Enzymes solution should be stored at 2-8 °C. The solution is stable for up to 1 month unless turbidity develops, and for at least 6 months at –20 °C.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Effect of dehydration methods on digested starch fractions of retrograded debranched rice starch
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