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P8333

Sigma-Aldrich

Anti-Protein Kinase Cδ antibody produced in rabbit

whole antiserum

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Synonym(s):
Anti-PKC δ
MDL number:
NACRES:
NA.44

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

rat

technique(s)

dot blot: 1:50,000
microarray: suitable
western blot: 1:10,000 using rat brain extract

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

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P4334SAB4502360SAB1412060
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

whole antiserum

antibody form

whole antiserum

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

species reactivity

rat

species reactivity

mouse, rat

species reactivity

human, mouse, rat

species reactivity

human

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General description

Protein Kinase C (PKC) is a family of serine/threonine (Ser/Thr)-specific protein kinases. PKC is a phospholipid-dependent enzyme, activated by the lipid 1,2-diacylglycerol (DAG). The protein kinase C delta (PKC δ) isoenzyme appears to be widely expressed in the brain, lung, heart, spleen, liver, ovary, pancreas, thymus, adrenal gland, skin and rat embryonic fibroblasts, and is expressed in lower levels in certain mouse fibroblasts. PKD is also located in the cytosol, nuclear compartment and in mitochondria in response to cellular stress.

Specificity

Anti-Protein Kinase C δ specifically reacts in dot-blot immunoassay with PKC δ peptide conjugated to BSA with 1-ethyl-3-(3-dimethylamino-propyl)-carbodiimide (EDCI).

Immunogen

Synthetic peptide corresponding to the C-terminal variable (V5) region (amino acids 662-673) of rat PKC δ coupled to KLH with glutaraldehyde.

Application

Anti- protein kinase c δ antibody may be used in:
  • immunoprecipitation
  • immunohistochemistry
  • immunoblotting
  • ELISA
  • chemiluminescence detection systems to detect PKC δ
  • dot-blot immunoassay
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Protein Kinase C isotype δ (PKCδ) modulates the inflammatory response. It acts as a signal transducer of several signaling pathways. Hence it can be considered as a vital therapeutic target to treat sepsis induced-lung injury. In sepsis, PKCδ participates in platelet-mediated activation. Overexpression and stimulation of PKC δ leads to cell division arrest in Chinese hamster ovary (CHO) cells and growth inhibition of NIH3T3 cells.
Protein kinase C (PKC) has a pivotal role in cell growth and differentiation, modulation of neurotransmission, signal transduction and oncogenesis. Anti-protein kinase c δ antibody can be used for studying the differential tissue expression and intracellular localization of PKC δ. It can also be used in western blotting and microarray.

Physical form

Rabbit Anti-Protein Kinase C δ is supplied as liquid containing 0.1% sodium azide as preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended.Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Role of Protein Kinase C-delta in regulating platelet activation and platelet-leukocyte interaction during sepsis
Liverani E, et al.
Testing, 13(4), e0195379-e0195379 (2018)
Protein kinase D activation induces mitochondrial fragmentation and dysfunction in cardiomyocytes
Jhun BS, et al.
The Journal of Physiology, 596(5), 827-855 (2018)
Ikuko Koyama-Honda et al.
Autophagy, 9(10), 1491-1499 (2013-07-26)
Autophagosome formation is governed by sequential functions of autophagy-related (ATG) proteins. Although their genetic hierarchy in terms of localization to the autophagosome formation site has been determined, their temporal relationships remain largely unknown. In this study, we comprehensively analyzed the
Peidu Jiang et al.
Molecular biology of the cell, 25(8), 1327-1337 (2014-02-21)
Membrane fusion is generally controlled by Rabs, soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), and tethering complexes. Syntaxin 17 (STX17) was recently identified as the autophagosomal SNARE required for autophagosome-lysosome fusion in mammals and Drosophila. In this study, to better
Pilar Almela et al.
Frontiers in pharmacology, 4, 164-164 (2014-01-11)
G protein-coupled receptors (GPCRs) comprise a large family of membrane receptors involved in signal transduction. These receptors are linked to a variety of physiological and biological processes such as regulation of neurotransmission, growth, and cell differentiation among others. Some of

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