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P9029

Sigma-Aldrich

o-Phenylenediamine

Peroxidase substrate, ≥98.0%, powder

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Synonym(s):
1,2-Diaminobenzene, 1,2-Phenylenediamine, OPD
Empirical Formula (Hill Notation):
C6H8N2
CAS Number:
Molecular Weight:
108.14
Beilstein/REAXYS Number:
606074
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.83

vapor density

3.7 (vs air)

Quality Level

vapor pressure

0.01 mmHg ( 25 °C)

assay

≥98.0%

form

powder

color

white to off-white

bp

256-258 °C

mp

98-102 °C

solubility

ethanol: 50 mg/mL, clear, colorless to faintly yellow

fluorescence

λex 337 nm; λem 417 nm (Pyruvic acid derivative)

storage temp.

2-8°C

SMILES string

Nc1ccccc1N

InChI

1S/C6H8N2/c7-5-3-1-2-4-6(5)8/h1-4H,7-8H2

InChI key

GEYOCULIXLDCMW-UHFFFAOYSA-N

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1 of 4

This Item
P5412P23938694975
o-Phenylenediamine Peroxidase substrate, ≥98.0%, powder

P9029

o-Phenylenediamine

o-Phenylenediamine tablet, 20 mg substrate per tablet

P5412

o-Phenylenediamine

o-Phenylenediamine flaked, 99.5%

P23938

o-Phenylenediamine

o-Phenylenediamine sublimed, ≥99%

694975

o-Phenylenediamine

solubility

ethanol: 50 mg/mL, clear, colorless to faintly yellow

solubility

-

solubility

-

solubility

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

form

powder

form

tablet

form

powder

form

sublimed

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

-

color

white to off-white

color

-

color

-

color

-

General description

o-Phenylenediamine (OPD) Free Base is a peroxidase substrate suitable for use in enzyme linked immunosorbent assay (ELISA) procedures. The substrate produces a soluble product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Application

o-Phenylenediamine has been used in the microtiter?based assay for hyaluronan quantification. It has also been used in enzyme linked immunosorbent assay (ELISA) for color development.

Caution

May darken in storage.

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

276.8 °F - closed cup

flash_point_c

136 °C - closed cup


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Articles

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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