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PLA0029

Sigma-Aldrich

Rabbit anti-BLM Antibody, Affinity Purified

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Synonym(s):
BS, Bloom syndrome, DNA helicase, RECQ2, RECQL2, RECQL3, RecQ helicase-like, RecQ-like type 2, recQ protein-like 3
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

grade

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species reactivity

human, mouse

technique(s)

immunoprecipitation (IP): 2-10 μg/mg
western blot: 1:1,000- 1:10,000

accession no.

NP_000048.1

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

rabbit ... BLM(641)

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This Item
HPA005689SAB4502734WH0000641M1
species reactivity

human, mouse

species reactivity

human

species reactivity

human

species reactivity

human

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

mouse

technique(s)

immunoprecipitation (IP): 2-10 μg/mg, western blot: 1:1,000- 1:10,000

technique(s)

immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:50-1:200

technique(s)

ELISA: 1:1000, immunofluorescence: 1:100-1:500, immunohistochemistry: 1:50-1:100

technique(s)

immunofluorescence: suitable, indirect ELISA: suitable, western blot: 1-5 μg/mL

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

UniProt accession no.

P54132

UniProt accession no.

P54132

UniProt accession no.

P54132

UniProt accession no.

P54132

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Immunogen

The epitope recognized by PLA0029 maps to a region between residue 1325 and the C-terminus (residue 1417) of human Bloom Syndrome using the numbering given in entry NP_000048.1 (GeneID 641).

Physical form

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Other Notes

Bloom syndrome protein (BLM) is a RecQ DNA-helicase that plays a role in DNA replication and repair. Mutations and inactivation of BLM result in human syndromes characterized by dwarfism, infertility, erythema, learning disabilities, immune deficiency and predisposition to cancer. BLM has been shown to associate with the BRCA1-associated genome surveillance complex (BASC) which contains BRCA1, MSH2, MSH6, MLH1, ATM, PMS2 and the RAD50-MRE11-NBS1 protein complex.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Emily Yun-Chia Chang et al.
The Journal of cell biology, 216(12), 3991-4005 (2017-10-19)
Sgs1, the orthologue of human Bloom's syndrome helicase BLM, is a yeast DNA helicase functioning in DNA replication and repair. We show that SGS1 loss increases R-loop accumulation and sensitizes cells to transcription-replication collisions. Yeast lacking SGS1 accumulate R-loops and
Prasun Chakraborty et al.
Nature communications, 12(1), 4126-4126 (2021-07-07)
Double stranded DNA Breaks (DSB) that occur in highly transcribed regions of the genome are preferentially repaired by homologous recombination repair (HR). However, the mechanisms that link transcription with HR are unknown. Here we identify a critical role for DHX9

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