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RAB0018

Sigma-Aldrich

Apelin EIA Kit

for serum, plasma, culture supernatant and cell lysates

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Synonym(s):
Apelin
NACRES:
NA.32

species reactivity

rat, mouse, human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
enzyme immunoassay: suitable

input

sample type cell lysate
sample type plasma
sample type serum
sample type culture supernatant(s)

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 5.84 pg/mL
standard curve range: 0.1-1000 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... APLN(8862)

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RAB0018

Apelin EIA Kit

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Adiponutrin EIA Kit

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RAB0326

C-Peptide EIA Kit

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

input

sample type cell lysate
sample type plasma
sample type serum
sample type culture supernatant(s)

input

sample type cell lysate
sample type plasma
sample type culture supernatant(s)
sample type serum

input

sample type plasma
sample type cell lysate
sample type culture supernatant(s)
sample type serum

input

sample type serum
sample type cell lysate
sample type plasma
sample type culture supernatant(s)

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 5.84 pg/mL
standard curve range: 0.1-1000 ng/mL

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 21 pg/mL
standard curve range: 0.1-1000 ng/mL

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 772 pg/mL
standard curve range: 0.1-1000 ng/mL

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.02 pg/mL
standard curve range: 0.1-1000 pg/mL

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

The Apelin C-Terminus Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Apelin C-Terminus peptide based on the principle of Competitive Enzyme Immunoassay. The EIA-APC-1 kit detects all active Apelin peptides, including Apelin-13, Apelin-28, Apelin-31 and Apelin-36.

Immunogen

Apelin, synthetic peptide

Application

For research use only. Not for use in diagnostic procedures.
Apelin EIA kit has been used to determine the concentration of apelin in pre-adipocyte growth medium.

Biochem/physiol Actions

Apelin (APLN) is secreted by white adipose tissue. It is an adipokine which has a role in tumor cell progression and vascularization. The protein may have a role in obesity-related disorders. APLN is involved in modulating energy homeostasis, water balance, glucose metabolism and immunity. It has been shown to be upregulated in many cancers.

Kit Components Also Available Separately

Product No.
Description
SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 2


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Bogusz Trojanowicz et al.
Toxins, 12(12) (2020-12-02)
Apelin peptides (APLN) serve as second substrates for angiotensin-converting enzyme 2 (ACE2) and, in contrast to angiotensin II (AngII), exert blood-pressure lowering and vasodilatation effects through binding to G-coupled APLN receptor (APLNR). ACE2-mediated cleavage of the APLN may reduce its
Apelin Attenuates Oxidative Stress in Human Adipocytes*
Aung Than
The Journal of Biological Chemistry (2014)
Assessment of Apelin Serum Levels in Persistent Atrial Fibrillation and Coronary Artery Disease.
Riazian M
The American Journal of the Medical Sciences (2016)
Serum apelin levels and body composition changes in breast cancer patients treated with an aromatase inhibitor.
Salman T
Journal of B.U.ON. : Official Journal of the Balkan Union of Oncology (2016)
Search for Genetic Variant in the Apelin Gene by Resequencing and Association Study in European Subjects.
Sentinelli F
Genetic Testing and Molecular Biomarkers (2016)

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