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RAB0361

Sigma-Aldrich

Human MMP-1 ELISA Kit

for serum, plasma, cell culture supernatant and urine

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Synonym(s):
MMP-1, Matrix metalloproteinase-1
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 8 pg/mL
standard curve range: 24.69-18000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MMP1(4312)

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Human MMP-1 ELISA Kit for serum, plasma, cell culture supernatant and urine

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Human MMP-10 ELISA Kit

Human MMP-3 ELISA Kit for serum, plasma, cell culture supernatant and urine

RAB0367

Human MMP-3 ELISA Kit

Gene Information

human ... MMP1(4312)

Gene Information

human ... MMP1(4312)

Gene Information

human ... MMP10(4319)

Gene Information

human ... MMP3(185250)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 8 pg/mL
standard curve range: 24.69-18000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 8 pg/mL
standard curve range: 24.69-18000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 2.06-1500 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 300 pg/mL
standard curve range: 0.41-100 ng/mL

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum
sample type urine

input

sample type tissue lysate
sample type cell lysate

input

sample type urine
sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type cell culture supernatant(s)
sample type urine
sample type serum
sample type plasma

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

General description

The Human MMP-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP-1 pro and active forms in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human MMP1

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human MMP-1 ELISA Kit has been used in ELISA assays.

Biochem/physiol Actions

Matrix metalloproteinases (MMPs) enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-1 is a secreted collagenase with specificity toward Type I, II, III, VII, and X collagens, aggrecan, serpins, and α2-macroglobulin. It is overexpressed in lumbar intervertebral disc degeneration (IDD). It might also be associated with osteoarthritis. MMP-1 is linked with juvenile idiopathic arthritis. Genetic variations in MMP-1 can affect the susceptibility and severity of this disease.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8B - Non-combustible, corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Influence of Matrix metalloproteinase 1 and 3 genetic variations on susceptibility and severity of juvenile idiopathic arthritis.
Abd-Allah SH, et. al.
IUBMB Life, 67, 934-942 (2015)
The development of a matrix metalloproteinase-1 biosensor based on the surface plasmon resonance imaging technique
Tokarzewicz A, et al.
Analytical Methods : Advancing Methods and Applications, 8(34), 428-6435 (2016)
Expression profiles of MMP-1 and TIMP-1 in lumbar intervertebral disc degeneration.
Deng B, et. al.
Genetics and molecular research : GMR, 14, 19080-19086 (2015)
Regulation of matrix metalloproteinase expression in tumor invasion.
Westermarck J and Kahari VM
FEBS Journal, 13, 781-792 (1999)
MMP-2 functions as an early response protein in ovarian cancer metastasis.
Kenny HA, et. al.
Cell Cycle, 8, 683-688 (2009)

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