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S0677

Sigma-Aldrich

Streptavidin from Streptomyces avidinii

recombinant, expressed in E. coli, lyophilized powder

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CAS Number:
MDL number:
NACRES:
NA.46

recombinant

expressed in E. coli

form

lyophilized powder

capacity

4 mol/mol binding capacity (Biotin)

storage temp.

−20°C

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This Item
85878A2901189730
Sigma-Aldrich

Sigma-Aldrich

189730

Streptavidin

form

lyophilized powder

form

powder

form

lyophilized powder

form

lyophilized solid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

capacity

4 mol/mol binding capacity (Biotin)

capacity

-

capacity

-

capacity

-

General description

Streptavidin is a biotin-binding protein that is obtained from Streptomyces avidinii. It is resistant to extreme temperature, pH, detergents and proteolytic enzymes. Streptavidin has a molecular weight of 60kDa and exists as a homotetramer.

Application

Streptavidin from Streptomyces avidinii has been used-
  • for blocking of the AFM (atomic force microscopy) tip, during the measurement of unbinding forces in the avidin-biotin system
  • for coupling of anti-HA (hemagglutinin) antibodies during cell free synthesis and assembly of proteins on a biochip
  • for the coating of the gelatin flow phantoms to eventually determine the feasibility of IVUS (intravascular ultrasound) transducer for (biotinylated) microbubble-based drug delivery
  • to immobilize proteins and oligonucleotides

Biochem/physiol Actions

Streptavidin is an antibiotic that functions by binding to and depleting the essential vitamin biotin from the surrounding environment. Because of its unique properties, streptavidin has found various applications in biological studies, including immunotherapy, immunoassays, hybridization assays, lymphocyte activation, antigen localization and affinity chromatography.

Unit Definition

One unit will bind 1.0 μg biotin.

Physical form

Lyophilized from 5 mM potassium phosphate buffer

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Nicola Ilk et al.
Applied and environmental microbiology, 68(7), 3251-3260 (2002-06-29)
The nucleotide sequence encoding the crystalline bacterial cell surface (S-layer) protein SbpA of Bacillus sphaericus CCM 2177 was determined by a PCR-based technique using four overlapping fragments. The entire sbpA sequence indicated one open reading frame of 3,804 bp encoding
Identification of the Heparan Sulfate Binding Sites in the Cellular Prion Protein*
Richard G
The Journal of Biological Chemistry (2002)
John G Bruno et al.
Microchemical journal : devoted to the application of microtechniques in all branches of science, 115, 32-38 (2014-04-26)
Fifty-two candidate DNA aptamer sequences were selected for binding to the cardiovascular biomarker B-type or brain natriuretic peptide (BNP). Candidate aptamers were screened to rank their relative affinities against BNP by an aptamer-based ELISA-like aptamer microplate assay (ELASA). The highest
An IVUS transducer for microbubble therapies.
Kilroy JP et al
IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control, 61(3), 441-449 (2014)
Determination of binding curves via protein micropatterning in vitro and in living cells
Stefan S
Cytometry (2012)

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