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S4322

Sigma-Aldrich

Anti-Sirt6 (N-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-NAD-dependent deacetylase sirtuin 6, Anti-SIR2-like protein 6, Anti-SIR2L6
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~40 kDa

species reactivity

rat (predicted), mouse, human

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

western blot: 2-4 μg/mL using whole extracts of human 293 cells transfected with Sirt6
western blot: 2-4 μg/mL using whole extracts of mouse 3T3 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SIRT6(51548)
rat ... Sirt6(299638)

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This Item
S5947S4072S5322
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

species reactivity

rat (predicted), mouse, human

species reactivity

mouse (predicted), human

species reactivity

human

species reactivity

human

UniProt accession no.

Q8N6T7

UniProt accession no.

Q9NRC8

UniProt accession no.

Q9NTG7

UniProt accession no.

Q96EB6

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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General description

Sirtuin 6 (Sirt6) is a nuclear and chromatin-associated protein that belongs to the mammalian Sir2 gene family. It is broadly expressed with the highest levels in muscle, brain and heart.

Specificity

Anti-Sirt6 (N-terminal) recognizes human and mouse Sirt6. The detection of the Sirt6 band by immunoblotting is specifically inhibited with the immunizing peptide.

Immunogen

synthetic peptide corresponding to amino acids 19-33 of human Sirt6 via a C-terminal cysteine residue. The sequence is identical in mouse and differs by one amino acid in rat.

Application

Anti-Sirt6 (N-terminal) antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Sirtuin 6 (Sirt6) plays a key role in DNA repair and maintenance of genomic stability in cells. Sirt6 is enzymatically inactive as a NAD+ -dependent protein deacetylase but displays auto-ADP-ribosyltransferase activity. Loss of Sirt6 leads to the development of an acute degenerative aging-like phenotype.

Physical form

Solution in 0.01 M phosphate buffered, saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Differential expression of sirtuin family members in the developing, adult, and aged rat brain
Sidorova-Darmos E, et al.
Frontiers in Aging Neuroscience, 6 (2014)
Sergii Domanskyi et al.
Apoptosis : an international journal on programmed cell death, 22(11), 1336-1343 (2017-09-01)
Apoptosis is essential for numerous processes, such as development, resistance to infections, and suppression of tumorigenesis. Here, we investigate the influence of the nutrient sensing and longevity-assuring enzyme SIRT6 on the dynamics of apoptosis triggered by serum starvation. Specifically, we
Mouse Sir2 Homolog SIRT6 Is a Nuclear ADP-ribosyltransferase
Liszt G, et al.
Test, 280 (2005)
Protective effect of pyrroloquinoline quinine on ultraviolet A xn-irradiationinduced-fm9j human dermal fibroblast senescence in vitro proceeds via the xn-antiapoptotic-sm6g sirtuin 1/nuclear xn-factorderived-um6g erythroid xn-2related-9m3d factor 2/heme oxygenase 1 pathway
Zhang C, et al.
Molecular Medicine Reports, 12 (2015)
Justin W Nicholatos et al.
Acta neuropathologica communications, 6(1), 120-120 (2018-11-10)
Parkinson's disease is characterized by progressive death of dopaminergic neurons, leading to motor and cognitive dysfunction. Epidemiological studies consistently show that the use of tobacco reduces the risk of Parkinson's. We report that nicotine reduces the abundance of SIRT6 in

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