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S5569

Sigma-Aldrich

Anti-Superoxide Dismutase (MnSOD) (KC-19) antibody produced in rabbit

enhanced validation

IgG fraction of antiserum, buffered aqueous solution

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Synonym(s):
Anti-IPO-B, Anti-Indophenoloxidase B, Anti-MNSOD, Anti-Manganese Dependent SOD, Anti-Manganese Superoxide Dismutase 2, Anti-Mitochodrial SOD, Anti-Mn-SOD, Anti-SOD2, Anti-Superoxide Dismutase 2
MDL number:

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~24 kDa

species reactivity

rat, human

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

indirect immunofluorescence: 1:250-1:500 using methanol-acetone-fixed rat NRK cells
western blot: 1:1,000-1:2,000 using whole extract of rat brain and a chemiluminescent detection reagent
western blot: 1:2,000-1:4,000 using human HeLa mitochondria extract and a chemiluminescent detection reagent

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SOD2(6648)
mouse ... Sod2(20656)
rat ... Sod2(24787)

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This Item
S2147SAB2102261SAB5200083
biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

antibody form

IgG fraction of antiserum

antibody form

ascites fluid

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Gene Information

human ... SOD2(6648)
mouse ... Sod2(20656)
rat ... Sod2(24787)

Gene Information

human ... SOD1(6647)
rat ... Sod1(24786)

Gene Information

human ... SOD2(6648)

Gene Information

rat ... SOD1(24786)

mol wt

antigen ~24 kDa

mol wt

-

mol wt

24 kDa

mol wt

antigen predicted mol wt 23 kDa

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General description

Manganese superoxide dismutase (MnSOD) protein is a homotetrameric SOD. This protein is composed of four subunits, each containing one Mn2+. MnSOD enzyme is synthesized in the cytosol and is modified for its transport into the mitochondria where it resides in the matrix.
The SOD2 (superoxide dismutase 2) gene is mapped to human chromosome 6q25.3. The encoded protein functions as a tetramer and is localized to mitochondria.

Specificity

Anti-Superoxide Dismutase (MnSOD) (KC-19) recognizes human and rat Superoxide Dismutase (MnSOD).

Immunogen

synthetic peptide corresponding to amino acid residues 202-220 of human superoxide dismutase (MnSOD), conjugated to KLH. The corresponding sequence is identical in apes and monkeys, differs by one amino acid in mouse and horse, by two amino acids in cow, and by four amino acids in rat.

Application

Anti-Superoxide Dismutase (MnSOD) (KC-19) antibody produced in rabbit may be used in immunoblotting and indirect immunofluorescence.

Biochem/physiol Actions

Manganese superoxide dismutase (MnSOD) is essential for the survival of aerobic life. This protein is inducible by tumor necrosis factor (TNF) and protects cells from TNF-mediated apoptosis. MnSOD is proposed to function as a tumor suppressor by modulating the activity of redox-sensitive transcription factors and specific signal mediators. Overexpression of the MnSOD gene protects neurons from N-methyl-D-aspartate receptor (NMDA) and nitric oxide-induced neurotoxicity. A decline in the MnSOD activity occurs in aging, progeria, cancer, asthma and transplant rejection.
Superoxide Dismutase 2 (SOD2) is an antioxidant enzyme and catalyzes the conversion of superoxide to hydrogen peroxide and molecular oxygen. The enzyme offers protection against oxidative damage induced by reactive oxygen species and also is radioprotective. The gene is associated with the metastasis and invasion process of salivary adenoid cystic carcinoma. Manganese SOD is involved in detoxification.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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S K Manna et al.
The Journal of biological chemistry, 273(21), 13245-13254 (1998-05-28)
Several recently identified intracellular proteins associate with the tumor necrosis factor (TNF) receptor and activate nuclear transcription factor (NF)-kappaB, c-Jun kinase, and apoptosis. However, the mechanism is not understood. In the present report, we investigated the role of reactive oxygen
M Gonzalez-Zulueta et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 18(6), 2040-2055 (1998-04-18)
Neuronal nitric oxide synthase (nNOS) neurons kill adjacent neurons through the action of NMDA-glutamate receptor activation, although they remain relatively resistant to the toxic effects of NMDA and NO. The molecular basis of the resistance of nNOS neurons to toxic
L A Macmillan-Crow et al.
Free radical research, 34(4), 325-336 (2001-05-01)
Manganese superoxide dismutase (MnSOD) is essential for life as dramatically illustrated by the neonatal lethality of mice that are deficient in MnSOD. In addition, mice expressing only 50% of the normal compliment of MnSOD demonstrate increased susceptibility to oxidative stress
Demet Candas et al.
Antioxidants & redox signaling, 20(10), 1599-1617 (2013-04-16)
The mitochondrial antioxidant manganese superoxide dismutase (MnSOD) is encoded by genomic DNA and its dismutase function is fully activated in the mitochondria to detoxify free radical O2(•-) generated by mitochondrial respiration. Accumulating evidence shows an extensive communication between the mitochondria
Dražen Švagelj et al.
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 124(4), 252-256 (2016-01-15)
Human gastric diseases have shown significant changes in the activity and expression of superoxide dismutase (SOD) isoforms. The aim of this study was to detect Mn-SOD activity and expression in the tissue of gastric mucosa, primarily in chronic gastritis (immunohistochemical

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