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SAB4200080

Sigma-Aldrich

Anti-Vinculin antibody, Mouse monoclonal

clone V284, purified from hybridoma cell culture

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Synonym(s):
Anti-CMD1W, Anti-MVCL, Anti-VCL
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture
purified immunoglobulin

antibody product type

primary antibodies

clone

V284, monoclonal

form

buffered aqueous solution

mol wt

antigen ~117 kDa

species reactivity

rabbit, chicken, human, rat, monkey, mouse, canine

concentration

~1 mg/mL

technique(s)

immunocytochemistry: 4-8 μg/mL using HeLa cells fixed with methanol:acetone
immunoprecipitation (IP): suitable
western blot: 0.005-0.01 μg/mL using HeLa total cell extracts

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

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This Item
V9264SAB4200691V4505
antibody form

purified from hybridoma cell culture, purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

ascites fluid

species reactivity

rabbit, chicken, human, rat, monkey, mouse, canine

species reactivity

frog, chicken, mouse, canine, human, bovine, rat, turkey

species reactivity

guinea pig, porcine, bovine (proinsulin), rat, monkey, sheep, dog, horse, human (insulin ), rabbit

species reactivity

bovine, human, chicken, mouse

clone

V284, monoclonal

clone

hVIN-1, monoclonal

clone

K36AC10, monoclonal

clone

VIN-11-5, monoclonal

Gene Information

human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

Gene Information

human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

Gene Information

bovine ... Ins(280829)
dog ... Ins(483665)
guinea pig ... Ins(100379579)
horse ... Ins(111776000)
human ... INS(3630)
pig ... Ins(397415)
rabbit ... Ins(100009181)
rhesus monkey ... Ins(704534)

Gene Information

human ... VCL(7414)
mouse ... Vcl(22330)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

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General description

Monoclonal Anti-Vinculin (mouse IgG1 isotype) is derived from the hybridoma V284 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with purified human platelet vinculin. In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), neuromuscular junctions, myotendinous junctions (skeletal muscle) and the membrane-associated dense bodies of smooth muscle.
Vinculin (VCL) is a ubiquitously expressed cytoskeletal protein, encoded by the gene mapped to human chromosome 10q11.2-qter. VCL is a vital element of adherens type cell junctions. Vinculin structure contains a globular head connected to a tail domain by a short proline-rich sequence, and an intramolecular interaction between the head and tail hides the various ligand-binding sites in the protein.

Immunogen

purified human platelet vinculin.

Application

Anti-Vinculin antibody, Mouse monoclonal has been used:
  • to probe blots in immunoblotting
  • immunochemical staining
  • immunoprecipitation
  • immunocytochemistry

Monoclonal Anti-Vinculin antibody produced in mouse has been used in western blotting.

Biochem/physiol Actions

Vinculin (VCL) is a cytoskeletal protein that supports cell-to-cell and cell to matrix junctions. It is also essentially involved in normal embryonic development and cardiac function, due to its role in controlling adhesion and motility, as well as cell spreading. In mature focal adhesions (FAs), VCL acts as an important constituent of the “molecular clutch” that induces the transmission of force from cytoplasmic F-actin to membrane-bound integrins. Aberration or mutation of VCL leads to cardiomyopathy, which is associated with hypertension. experimental studies reveal that, VCL might be used as a biomarker of aging or heart failure. Vinculin associates with FAs and assists in several cellular functions, such as stabilizing and strengthening FAs and promoting their maturation, extracellular matrix (ECM) mechanosensing, regulating actin cytoskeletal dynamics and signaling pathways involved in apoptosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Vinculin, an intracellular protein localized at specialized sites where microfilament bundles terminate at cell membranes
Geiger B, et al.
Proceedings of the National Academy of Sciences of the USA, 77(7), 4127-4131 (1980)
Gerald S Braun et al.
Journal of the American Society of Nephrology : JASN, 27(1), 132-142 (2015-06-05)
The role of IL-6 signaling in renal diseases remains controversial, with data describing both anti-inflammatory and proinflammatory effects. IL-6 can act via classic signaling, engaging its two membrane receptors gp130 and IL-6 receptor (IL-6R). Alternatively, IL-6 trans-signaling requires soluble IL-6R
Paolo Benatti et al.
Oncotarget, 7(2), 1633-1650 (2015-12-10)
The trimeric transcription factor NF-Y binds to the CCAAT box, an element enriched in promoters of genes overexpressed in tumors. Previous studies on the NF-Y regulome identified the general term metabolism as significantly enriched. We dissect here in detail the
Esther N Pesciotta et al.
PloS one, 10(10), e0140036-e0140036 (2015-10-17)
Diamond Blackfan Anemia (DBA) is a rare, congenital erythrocyte aplasia that is usually caused by haploinsufficiency of ribosomal proteins due to diverse mutations in one of several ribosomal genes. A striking feature of this disease is that a range of
In-Depth, Label-Free Analysis of the Erythrocyte Cytoplasmic Proteome in Diamond Blackfan Anemia Identifies a Unique Inflammatory Signature
Pesciotta EN
PLoS ONE, 10 (2015)

Articles

Quantitative and qualitative western blotting to validate knockdown by esiRNA.

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