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SAB4200241

Sigma-Aldrich

Anti-PP2A subunit B isoform PR55-α antibody, Mouse monoclonal

clone 2G9, purified from hybridoma cell culture

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Synonym(s):
Anti-B55α, Anti-PPP2R2A, Anti-PR52A, Anti-PR55A, Anti-Protein phosphatase 2 (formerly 2A), regulatory subunit B (PR52), alpha isoform, Anti-Protein phosphatase 2, regulatory subunit B, alpha, B55A, Anti-Serine/threonine protein phosphatase 2A, 55 kDa regulatory subunit B, alpha isoform

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

2G9, monoclonal

form

buffered aqueous solution

mol wt

antigen 50-55 kDa

species reactivity

rat, human, mouse

concentration

~1.0 mg/mL

technique(s)

immunohistochemistry: suitable
indirect immunofluorescence: suitable
western blot: 1-2 μg/mL using whole extracts of mouse 3T3, rat NRK or human HeLa cells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

General description

Monoclonal Anti-PP2A subunit B isoform PR55- (mouse IgG1 isotype) is derived from the hybridoma 2G9 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of rat PP2A subunit B isoform PR55-. PP2A subunit B isoform PR55- (PPP2R2A) belongs to the phosphatase (PP)2A regulatory subunit B family. Protein phosphatase 2A is one of the major Ser/Thr phosphatases. PPP2R2A encodes an alpha isoform of the regulatory subunit B55 subfamily. This gene is mapped to human chromosome 8p21.2.

Specificity

Monoclonal Anti- PP2A subunit B isoform PR55-α binds to rat, human and mouse PP2A subunit B isoform PR55-α. The antibody may also react with rat PP2A-B55-δ, human PP2A-B55-β and weakly associates with rabbit PP2A-B55-γ.

Application

Monoclonal Anti-PP2A subunit B isoform PR55-α antibody produced in mouse has been used in:
  • immunoblotting
  • immunohistochemistry
  • immunofluorescence

Biochem/physiol Actions

PP2A is a serine/threonine phosphatase that downregulates the MAP kinase pathway and subsequently modulates mitogenic signaling. This phosphatase also mediates cellular invasion, localization of Sgo1 at centromeres, cell movement, Ras-1 activation, and chromosome segregation. PP2A protein complex consists of a dimeric core containing subunits A and C; which in turn associates with the regulatory subunit B. PP2A subunit B is involved in the degranulation of mast cells . Monoclonal Anti- PP2A subunit B isoform PR55-α binds to rat, human and mouse PP2A subunit B isoform PR55-α. The antibody may also react with rat PP2A-B55-δ, human PP2A-B55-β and weakly associates with rabbit PP2A-B55-γ.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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