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SAB4200249

Sigma-Aldrich

Anti-PTPN2 antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody

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Synonym(s):
Anti-PTN2, Anti-PTPT, Anti-TC-PTP, Anti-TCELLPTP, Anti-TCPTP
NACRES:
NA.44

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~48 kDa

species reactivity

mouse, rat, human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.5 mg/mL

technique(s)

western blot: 1.5-3.0 μg/mL using Jurkat cell extracts, rat kidney extracts (S1 fraction) or mouse kidney extracts (S1 fraction).

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PTPN2(5771)

General description

Protein tyrosine phosphatase non-receptor type 2 (PTPN2), also known as T cell protein tyrosine phosphatase (TCPTP), is an intracellular nonreceptor tyrosine-specific phosphatase that is expressed ubiquitously at various stages of mammalian development. PTPN2 exists as two alternatively spliced variants, PTPN2 isoform 1 (p48TC, 48 kDa) that is targeted to the endoplasmic reticulum by a hydrophobic C-terminal region and a shorter PTPN2 isoform 2 (p45TC, 45 kDa) that is targeted to the nucleus.

Immunogen

synthetic peptide corresponding to a sequence located near the C-terminus of human PTPN2, conjugated to KLH. The corresponding sequence is identical in human PTPN2 isoform 2 and highly conserved (single amino acid substitution) in mouse PTPN2.

Application

Anti-PTPN2 antibody produced in rabbit has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunostaining

Biochem/physiol Actions

PTPN2 acts on several cytoplasmic substrates including epidermal growth factor receptor (EGFR), insulin receptor (InsR), Shc, janus kinases (JAKs), and the nuclear substrate signal transducer and activator of transcription 1 (STAT1). PTPN2 has been associated with type 1 diabetes.
Protein tyrosine phosphatase non-receptor type 2 (PTPN2) is a tyrosine phosphatase that negatively regulates tyrosine kinase and JAK/STAT signaling pathways. PTPN2 inhibits oncogenic JAK1 and thereby functions as a tumor suppressor in T-cell malignancies. Genetic alterations in PTPN2 have been associated with the risk of Crohn′s disease and ulcerative colitis .

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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TC-PTP directly interacts with connexin43 to regulate gap junction intercellular communication
Li H, et al.
Journal of Cell Science, 127(15), 3269-3279 (2014)
T-cell protein tyrosine phosphatase (TCPTP) is irreversibly inhibited by etoposide-quinone, a reactive metabolite of the chemotherapy drug etoposide
Qing N, et al.
Molecular Pharmacology (2019)
Protein tyrosine phosphatases and type 1 diabetes: genetic and functional implications of PTPN2 and PTPN22
Cerosaletti K and Buckner JH
The Review of Diabetic Studies : RDS, 9(4), 188-188 (2012)
Protein tyrosine phosphatase non-receptor type 2 and inflammatory bowel disease
Spalinger MR, et al.
World Journal of Gastroenterology, 22(3), 1034-1034 (2016)
Hanjun Li et al.
Journal of cell science, 127(Pt 15), 3269-3279 (2014-05-23)
Protein kinases have long been reported to regulate connexins; however, little is known about the involvement of phosphatases in the modulation of intercellular communication through gap junctions and the subsequent downstream effects on cellular processes. Here, we identify an interaction

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