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SAB4200255

Sigma-Aldrich

Anti-PP2A subunit B isoform B56-δ antibody, Mouse monoclonal

clone H5D12, purified from hybridoma cell culture

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Synonym(s):
Anti-PP2A B subunit isoform PR61-δ, Anti-PP2A B subunit isoform R5-δ, Anti-PPP2R5D, Anti-Protein phosphatase 2, regulatory subunit B′,δ, Anti-Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, δ isoform
NACRES:
NA.44

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

H5D12, monoclonal

form

buffered aqueous solution

mol wt

antigen ~72 kDa

species reactivity

human, rat

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): suitable
indirect immunofluorescence: suitable
western blot: 2.5-5.0 μg/mL using whole extracts of human U-2-OS or rat Rat2 cells.

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PPP2R5D(5528)

General description

Monoclonal Anti-PP2A subunit B isoform B56-δ (mouse IgG1 isotype) is derived from the hybridoma H5D12 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein. PP2A subunit B isoform B56 d (PPP2R5D) belongs to the phosphatase PP2A regulatory subunit B family. PP2A subunit B isoform B56 d (PPP2R5D) encodes a δ isoform of the regulatory subunit B56 subfamily. It is a member of phosphatase PP2A regulatory subunit B family. The core enzyme is a dimer composed of a catalytic subunit (C) and a constant regulatory subunit (A), that associates with a regulatory subunit (B).

Specificity

The antibody recognizes human and rat PP2A B subunit isoform B56-δ. The antibody does not cross-react with PP2A-B55, PP2A-B56-α, PP2A-B56-β, PP2A-B56-γ or PP2A-B56-ε.

Immunogen

fusion protein corresponding to the N-terminus of human PP2A B subunit isoform B56δ.

Application

Monoclonal Anti-PP2A subunit B isoform B56-δ antibody produced in mouse has been used in immunoblotting.

Biochem/physiol Actions

PP2A is a serine/threonine phosphatase that downregulates the MAP kinase pathway and subsequently modulates mitogenic signaling. This phosphatase also mediates cellular invasion, localization of Sgo1 at centromeres, cell movement, Ras-1 activation, and chromosome segregation. PP2A protein complex consists of a dimeric core containing subunits A and C; which in turn associates with the regulatory subunit B. PP2A subunit B is involved in the degranulation of mast cells .
Protein phosphatase 2A (PP2A) is implicated in the negative control of cell growth and division.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Regulation of the Cell Cycle by Protein Phosphatase 2A in Saccharomyces cerevisiae
Jiang Y
Microbiology and Molecular Biology Reviews, 70, 440-440 (2006)
Sunanda Basu
Current protein & peptide science, 12(1), 3-11 (2010-12-31)
Cell motility is a very critical phenomenon that plays an important role in the development of eukaryotic organisms. One of the well studied cell motility phenomena is chemotaxis, which is described as a directional movement of cell in response to
Gregory Kranias et al.
Cellular signalling, 22(12), 1882-1890 (2010-08-07)
Asthma is characterised by antigen-mediated mast cell degranulation resulting in secretion of inflammatory mediators. Protein phosphatase 2A (PP2A) is a serine/threonine protein phosphatase composed of a catalytic (PP2A-C) subunit together with a core scaffold (PP2A-A) subunit and a variable, regulatory
D Abraham et al.
The Journal of biological chemistry, 275(29), 22300-22304 (2000-05-10)
The Raf-1 kinase plays a key role in relaying proliferation signals elicited by mitogens or oncogenes. Raf-1 is regulated by complex and incompletely understood mechanisms including phosphorylation. A number of studies have indicated that phosphorylation of serines 259 and 621
Sean T DeGrande et al.
The Journal of biological chemistry, 288(2), 1032-1046 (2012-12-04)
Kinase/phosphatase balance governs cardiac excitability in health and disease. Although detailed mechanisms for cardiac kinase regulation are established, far less is known regarding cardiac protein phosphatase 2A (PP2A) regulation. This is largely due to the complexity of the PP2A holoenzyme

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