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SAB4200621

Sigma-Aldrich

Monoclonal Anti-NG2/CSPG4 antibody produced in mouse

clone 2164B6, purified from hybridoma cell culture

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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

2164B6, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

~1 mg/mL

technique(s)

flow cytometry: 2.5-5 μg/test using SK-Mel-28 cells.
immunofluorescence: 2.5-5 μg/mL using A375 cells.

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CSPG4(1464)

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This Item
SAB4200660SAB5700198SAB4200634
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

clone

2164B6, monoclonal

clone

GOL3-1

clone

polyclonal

clone

GR4-1, monoclonal

species reactivity

human

species reactivity

human, mouse, rat

species reactivity

mouse, rat, human

species reactivity

human, monkey

Gene Information

human ... CSPG4(1464)

Gene Information

human ... GOLPH3(64083)
mouse ... Golph3(66629)
rat ... Golph3(78961)

Gene Information

human ... CSPG4(1464)

Gene Information

human ... GRAMD4(23151)
mouse ... Gramd4(223752)

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

mouse

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General description

Nerve/glial antigen 2 (NG2), a high molecular mass melanoma- associated antigen (HMW-MAA), is a large integral membrane, cell surface type I transmembrane protein that is covalently modified with chondroitin sulfate glycosaminoglycan.

Immunogen

a recombinant extracellular portion of human NG2 proteoglycan

Application

Monoclonal Anti-NG2/CSPG4 antibody produced in mouse used in flow cytometry and immunocytochemistry.

Biochem/physiol Actions

Nerve/glial antigen 2 (NG2) plays a central role in linking multiple oncogenic pathways required for malignant progression. NG2 expression in radial growth phase (RGP) human melanomas enables migration, protease activation and epithelial to mesenchymal transition, which is important in primary tumors for facilitating progression from a radial to vertical growth phase phenotype. It regulates sustained, high-level activation of key survival and growth pathways, integrin-regulated focal adhesion kinase (FAK), extracellular signal-regulated kinases 1 and 2 (ERK 1,2) and phosphoinositide 3-kinase (PI3K)/AKT pathways.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Megan S Lord et al.
The FEBS journal, 280(10), 2490-2510 (2013-02-20)
Proteoglycans are ubiquitous dynamic molecules that are made up of a protein core to which specific linear glycosylation structures, known as glycosaminoglycans, have been covalently coupled. They have roles in many biological and pathological processes, which have been shown to
Matthew A Price et al.
Pigment cell & melanoma research, 24(6), 1148-1157 (2011-10-19)
Chondroitin sulfate proteoglycan 4 (CSPG4), a transmembrane proteoglycan originally identified as a highly immunogenic tumor antigen on the surface of melanoma cells, is associated with melanoma tumor formation and poor prognosis in certain melanomas and several other tumor types. The

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