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SAB4200755

Sigma-Aldrich

Anti-Human IgE-Peroxidase antibody, Mouse monoclonal

clone GE-1, purified from hybridoma cell culture

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Synonym(s):
Anti-Immunoglubulin E
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

peroxidase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

GE-1, monoclonal

form

lyophilized powder

species reactivity

human

technique(s)

ELISA: 1:20,000-1:30,000 using 5 μg/mL Human IgE myeloma for coating

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the GE-1 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgE myeloma protein. IgE (Immunoglobulin E) is the least abundant immunoglobulin isotype. IgE is produced by plasma cells and is highly functional in the lung, skin, and mucous membranes. Normal levels of IgE in serum are less than 1 μmL.

Specificity

Monoclonal Anti-Human IgE specifically recognizes Human IgE. The antibody shows no cross-reactivity with human IgG1, IgG2, IgG3, IgG4, IgA, and IgM.

Immunogen

Purified human IgE

Application

Anti-Human IgE-Peroxidase antibody has been used in enzyme linked immunosorbent assay (ELISA).

Biochem/physiol Actions

IgE plays a critical role in allergic reactions and host protection from parasites. During an allergic reaction such as atopic eczema, extrinsic asthma or hay fever, fungal and parasitic infection, atopic disease, or primary immunodeficiency the serum IgE levels can be induced up to 5-fold. IgE specifically and reversibly binds to the membrane receptor Fc-epsilon receptors (FcεR) type I on the surface of basophils, eosinophils, and mast cells. This binding activates the release of histamine, leukotrienes, and other mediators responsible for hypersensitivity. The recognition and isolation of allergen-specific IgE can lead to progress in understanding of pathophysiologic mechanisms underlying IgE-associated allergic disorders.

Physical form

Supplied as a lyophilized powder.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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