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SAB4200790

Sigma-Aldrich

Anti-Human IgG (Fc specific)-Peroxidase antibody, Mouse monoclonal

clone GG-7, purified from hybridoma cell culture

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Synonym(s):
Anti-Human immunoglobulin G
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

peroxidase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

GG-7, monoclonal

form

lyophilized powder

species reactivity

human

technique(s)

direct ELISA: 1:40,000-1:80,000 using 2.5 μg/mL human IgG1 for coating

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
SAB4200768SAB4200755SAB4200713
biological source

mouse

biological source

mouse

biological source

mouse

biological source

-

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

-

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

clone

GG-7, monoclonal

clone

8c/6-39, monoclonal

clone

GE-1, monoclonal

clone

GG-5, monoclonal

technique(s)

direct ELISA: 1:40,000-1:80,000 using 2.5 μg/mL human IgG1 for coating

technique(s)

ELISA: 1:8,000-1:16,000 using 5 μg/ml human IgG1 for coating

technique(s)

ELISA: 1:20,000-1:30,000 using 5 μg/mL Human IgE myeloma for coating

technique(s)

indirect ELISA: 0.25-0.5 μg/mL using 5μg/mL human IgG for coating

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General description

IgGs are the most common Immunoglobulins isotype in blood, lymph fluid, cerebrospinal fluid, and peritoneal fluid and a key players in the humoral immune response. IgGs include four subclasses (IgG1, IgG2, IgG3, and IgG4), they consist of a variable Fab fragment (which includes the antibody recognition site), and a conserved Fc fragment. The IgG subclasses differ in their physical and chemical properties, their distribution pattern is found to be age-dependent and every subclass has a specific biological function. IgG deficiencies are often associated with various diseases.

The Fc fragment has various important functions such as complement fixation, site for rheumatoid factor (autoantibody directed to Fc) attachment, passage through the placental membrane and protein A binding. A certain population of lymphocytes also possess an "Fc receptor". These functions indicate the importance of immunoreagents specific for the Fc fragment of Human IgG.

Monoclonal Anti-Human IgG (Fc specific) is specific for the Fc fragment of Human IgG and is observed with all human IgG subclasses but not with the Fab fragment of human IgG, purified light chains, human IgA or human IgM.

Immunogen

Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads.

Application

The antibody is recommended to use in various immunological techniques, including ELISA.

Physical form

Supplied as a lyophilized powder

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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