MilliporeSigma
All Photos(1)

Documents

SAB4200855

Sigma-Aldrich

Anti-Cytokeratin Peptide 18 antibody, Mouse monoclonal

clone CY-90, purified from hybridoma cell culture

Sign Into View Organizational & Contract Pricing

Synonym(s):
Anti-CK18

antibody form

purified from hybridoma cell culture

Quality Level

antibody product type

primary antibodies

clone

CY-90, monoclonal

form

liquid

species reactivity

human

concentration

~1 mg/mL

technique(s)

immunoblotting: suitable
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human placenta sections

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
C8541F4772F0397
species reactivity

human

species reactivity

wide range

species reactivity

wide range

species reactivity

-

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

form

liquid

form

-

form

buffered aqueous solution

form

buffered aqueous solution

isotype

IgG1

isotype

IgG1

isotype

IgG1

isotype

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Intermediate filaments (IFs) are one of three major cytoskeletal filament assemblies in higher eukaryotes.6 Keratins (formerly known as “cytokeratins”) comprise of types I and II IF genes (given a total of 54 conserved genes) that are highly expressed primarily in epithelial cells hair and skin appendages. Cytokeratins are the largest subgroup of IF in mammals and are the predominant subtype of IFs in all epithelia.6,7 Cytokeratins form a 10-nm filaments comprised of type I and II cytokeratine heteropolymer.7

Specificity

Monoclonal Anti-Cytokeratin Peptide 18 antibody specifically recognizes cytokeratin peptide 18 in human cultured cell lines as well as human tissue extracts. The antibody also shows reactivity with samples from rat1 and monkey2 origin. The antibody reacts specifically with a wide variety of simple epithelia (e.g. intestine, respiratory and urinary systems, liver and glandular epithelia). It does not react with stratified squamous epithelia (e.g. esophagus or epidermis) or with non-epithelial cells.

Application

The antibody may be used in various immunochemical techniques including Immunoblotting (~45 kDa)3, Immunofluorescence1-2, Immunoprecipitation4 and Immunohistology5.

Biochem/physiol Actions

Cytokeratins provide the cells with structural support, resistance to chemical stresses and maintenance of a polarized cytoarchitecture.6 Each epithelial tissue has a specific expression pattern of cytokeratins, which makes them common and excellent pathology markers for tumor identification, classification and epithelial cell differentiation.6,7
Cytokeratin 18 is a member of the type I subfamily. Cytokeratin pair 8/18 forms the IF scaffold predominantly in simple epithelia such as the liver and pancreas. They have major role in the mechanical support of the cell, protein biosynthesis, protection from apoptosis, regulation of cell cycle progression, motility and organelle transport.3
Cytokeratins over and misexpression are linked to various diseases and more than 60 different disorders (termed as keratinopathies) have been linked to inherited cytokeratin changes. Cytokeratins (such as cytokeratin 18) serve as serum markers for malignant and non-neoplastic disorders and mutation of human cytokeratin 18 has also been associated with liver diseases.7

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative. Antibody Concentration: ~ 1.0 mg/mL

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service