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SAB4200871

Sigma-Aldrich

Anti-Pseudomonas aeruginosa Exotoxin A antibody, Mouse monoclonal

clone EXO-68, purified from hybridoma cell culture

Synonym(s):

ETA, PE, Pseudomonas Exotoxin A

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

antibody form

purified from hybridoma cell culture

Quality Level

clone

EXO-68

form

liquid

species reactivity

Pseudomonas aeruginosa

concentration

~1 mg/mL

technique(s)

ELISA: 2.5-5 μg/mL using Exotoxin A from Pseudomonas aeruginosa for coating.
immunoblotting: 0.125-0.25 μg/mL using exotoxin A from Pseudomonas aeruginosa.

isotype

IgM

storage temp.

−20°C

target post-translational modification

unmodified

General description

Pseudomonas aeruginosa is a rod shaped, gram negative, monoflagellated, aerobic to facultative anaerobe bacteria which commonly inhabits soil and aqueous environments.1,2 Pseudomonas Exotoxin A (PE) is the most potent virulence factor secreted by some strains of P. aeruginosa It is composed of three structural domains, N-terminal domain (I) responsible for the toxin binding to its host cell receptor, middle domain (II) has a role in toxin translocation across the membrane, and C-terminal domain (III) which has ADP-ribosylation activity.4,5

Specificity

Monoclonal Anti-Exotoxin A from Pseudomonas aeruginosa antibody specifically recognizes Exotoxin A from Pseudomonas aeruginosa (ETA, PE) and has no cross reactivity with staphylococcal enterotoxin A and B (SEA, SEB) and cholera toxin.

Application

The antibody may be used in various immunochemical techniques including Immunoblotting (70 kDa) and ELISA.

Biochem/physiol Actions

P.aeruginosa is considered an opportunistic human pathogen mainly causing disease in immunocompromised patients. It is especially fatal in cystic fibrosis (CF) patients, but also presents a major problem in chronic wounds, burn wounds and infection of implanted biomaterials such as catheters.3The genome of P. aeruginosa encodes a vast arsenal of virulence factors such as, Type 3 secretion system (T3SS), type 4 pilli and several secreted proteases, lipases and phospholipases.1 The Exotoxin A ADP-ribosylation activity inhibits host elongation factor 2 (EF2), and protein synthesis.1 Due to its toxin ADP-ribosylation activity PE is considered as a selective agent for the elimination of specific cell populations resulting in the irreversible shut down of protein synthesis leading to cell death. To reduce the adverse effects of natural PE, mutated PE was used in several attempts to develop recombinant toxin-antibody or cytokines fusion fragments for therapeutic application including cancer immunotherapy.5-9

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shaan L Gellatly et al.
Pathogens and disease, 67(3), 159-173 (2013-04-27)
Pseudomonas aeruginosa is a metabolically versatile bacterium that can cause a wide range of severe opportunistic infections in patients with serious underlying medical conditions. These infections are characterized by an intense neutrophilic response resulting in significant damage to host tissues
D J Hassett
Journal of bacteriology, 178(24), 7322-7325 (1996-12-01)
Pseudomonas aeruginosa produced alginate and elevated algD (encoding GDPmannose 6-dehydrogenase) transcription under strict anaerobic conditions, especially when using nitrate as a terminal electron acceptor. Purified alginate added to bacterial suspensions caused a decrease in growth, suggesting that alginate contributes to
R Hertle et al.
Infection and immunity, 69(11), 6962-6969 (2001-10-13)
Pseudomonas aeruginosa is the major infectious agent of concern for cystic fibrosis patients. Strategies to prevent colonization by this bacterium and/or neutralize its virulence factors are clearly needed. Here we characterize a dual-function vaccine designed to generate antibodies to reduce
I Pastan et al.
Science (New York, N.Y.), 254(5035), 1173-1177 (1991-11-22)
Recombinant toxins target cell surface receptors and antigens on tumor cells. They kill by mechanisms different from conventional chemotherapy, so that cross resistance to conventional chemotherapeutic agents should not be a problem. Furthermore, they are not mutagens and should not
Lawrence R Mulcahy et al.
Microbial ecology, 68(1), 1-12 (2013-10-08)
Pseudomonas aeruginosa is a ubiquitous organism that is the focus of intense research because of its prominent role in disease. Due to its relatively large genome and flexible metabolic capabilities, this organism exploits numerous environmental niches. It is an opportunistic

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