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SAB4300058

Sigma-Aldrich

Anti-phospho-ERBB2 (pTyr1221/pTyr1222) antibody produced in rabbit

affinity isolated antibody

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Synonym(s):
Anti-CD340 antibody produced in rabbit, Anti-HER-2 antibody produced in rabbit, Anti-HER-2/neu antibody produced in rabbit, Anti-HER2 antibody produced in rabbit, Anti-v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro / glioblastoma derived oncogene homolog (avian) antibody produced in rabbit
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

~185 kDa

species reactivity

human

concentration

1 mg/mL

technique(s)

indirect immunofluorescence: 1:100-1:200
western blot: 1:500-1:1000

isotype

IgG

immunogen sequence

(N-L-YP-YP-W)

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr1221/pTyr1222)

Gene Information

human ... ERBB2(2064)

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SAB4300350SAB4300061SAB4300063
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

species reactivity

human

species reactivity

human

species reactivity

rat, mouse, human

species reactivity

human, rat

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

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General description

Erb-b2 receptor tyrosine kinase 2 (ErbB2) or human epidermal growth factor receptor 2 (HER2), is encoded by the gene mapped to human chromosome 17. The encoded protein belongs to the family of epidermal growth factors. ErbB2 membrane protein is characterized with a cysteine-rich extracellular ligand-binding domain, a hydrophobic membrane spanning region, and an intracellular tyrosine kinase domain.This protein does not have a ligand, and it either forms heterodimers with other family members or homodimer with itself when expressed at very high levels, to get activated. ErbB2 is expressed at low level in normal tissues, but at high level in breast cancer tissues.

Immunogen

Peptide sequence around phosphorylation site of tyrosine1221/1222 (N-L-Y(p)-Y(p) -W), according to the protein ERBB2.

Biochem/physiol Actions

Erb-b2 receptor tyrosine kinase 2 (ErbB2) signaling pathway stimulates cell proliferation and survival in the majority of breast cancers. Thus, over expression of this protein causes breast cancer. Heterodimer complex of ErbB2 and phosphatidylinositide 3-kinase (PI3K) is the most potent stimulator of the PI3K/AKT anti-apoptosis pathway. Increased expression of ErbB2 is also observed in ovarian, colorectal, pancreatic, endometrial and gastric cancers. Trastuzumab, an antibody, works by binding to a domain in the external domain of ErbB2. This domain is missing in p95, a truncated form of ErbB2, and hence these cancer cells show resistance to trastuzumab. ErbB2 protein can be used as a prognostic marker and as a therapeutic option for gynecologic cancers.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Target description

Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin.

Physical form

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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HER2 expression beyond breast cancer: therapeutic implications for gynecologic malignancies.
English DP
Molecular Diagnosis & Therapy, 17, 85-99 (2013)
HER2: biology, detection, and clinical implications.
Gutierrez C and Schiff R.
Archives of Pathology & Laboratory Medicine, 135, 55-62 (2011)
The role of p95HER2 in trastuzumab resistance in breast cancer.
Ozkavruk Eliyatkin N
Journal of B.U.ON. : Official Journal of the Balkan Union of Oncology, 21, 382-389 (2016)

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