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SAB4300470

Sigma-Aldrich

Anti-CTNNB1 (Ab-33) antibody produced in rabbit

affinity isolated antibody

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Synonym(s):
Anti-CTNNB antibody produced in rabbit, Anti-DKFZp686D02253 antibody produced in rabbit, Anti-FLJ25606 antibody produced in rabbit, Anti-FLJ37923 antibody produced in rabbit, Anti-catenin (cadherin-associated protein), beta 1, 88kDa antibody produced in rabbit
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

~92 kDa

species reactivity

human, rat, mouse

concentration

1 mg/mL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100
indirect immunofluorescence: 1:100-1:200
western blot: 1:500-1:1000

isotype

IgG

immunogen sequence

(L-D-S-G-I)

NCBI accession no.

UniProt accession no.

application(s)

research pathology

shipped in

wet ice

Storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CTNNB1(1499)

General description

Catenin beta-1 (CTNNB1) encoding β-catenin, belongs to armadillo-repeat family. In human chromosome, the gene CTNNB1 is localized on 3p22.1.

Immunogen

Peptide sequence around aa. 31-35 (L-D-S-G-I), according to the protein CTNNB1.

Biochem/physiol Actions

Catenin beta-1 (CTNNB1) is a signalling molecule in the nucleus and anchors actin cytoskeleton to cadherin-based adherens junctions. Phosphorylation causes dissociation of β-catenin from E-cadherin and degradation by proteasome pathway. β-catenin is a key molecule in wingless/integrated (wnt) signalling. β-catenin forms a complex with kindlin 2 and T-cell factor 4 (TCF4) to regulate wnt target gene expression. Mutations in adenomatous polyposis coli (APC) and CTNNB1 gene affects β-catenin intracellular localization and stability, contributing to aberrant expression of wnt/β-catenin in malignant melanoma. β-catenin is a major oncoprotein, which downregulates death associated protein kinase 2 (Dapk2) and inhibits anoikis of cancer cells. β-catenin expression is suppressed and its downstream gene transcription function is regulated by a tumor suppressor protein fragile histidine triad (Fhit). High expression of mucin 1 (MUC1), translocates β-catenin out of the nucleus and inhibits β-catenin regulated cell proliferation. Somatic mutations of CTNNB1 in glycogen synthase kinase 3β−casein kinase 1 (GSK-3β−CK1) phosphorylation site results in loss of phosphorylation of β-catenin. This causes aberrant wnt-signalling and leads to adrenocortical adenomas and adrenocortical carcinoma.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Target description

Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.

Physical form

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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