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SAB4500949

Sigma-Aldrich

Anti-MART-1 antibody produced in rabbit

affinity isolated antibody

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Synonym(s):
Antigen LB39-AA, Antigen SK29-AA, MART-1, Melan-A protein, Melanoma antigen recognized by T-cells 1
MDL number:
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 13 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

ELISA: 1:40000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MLANA(2315)

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This Item
M5691HPA028580SAB3500034
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

concentration

~1 mg/mL

concentration

-

concentration

-

concentration

-

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General description

MART-1 (melanoma antigen recognized by T cells) is also termed as Melan-A. It is a type III membrane protein with a molecular weight of 22-24 kDa. It is present in the endoplasmic reticulum (ER), trans-Golgi network (TGN) and in melanosomes. MART-1 is a melanocyte lineage protein. This gene is mapped to human chromosome 9p24.1.
Melanoma-associated antigen recognized by T cells-1 (MART-1) (also known as Melan-A) is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. It is a transmembrane protein which is hydrophobic in nature. Six other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, Tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. The protein is also expressed in melanoma tumors. Anti-MART-1 Antibody detects endogenous levels of total MART-1 protein.

Immunogen

The antiserum was produced against synthesized peptide derived from human MART-1.

Immunogen Range: 41-90

Biochem/physiol Actions

MART-1 (melanoma antigen recognized by T cells) is essential for the activities of the melanosomal matrix protein PMEL17 (premelanosome protein)/GP100 and the maturation of melanosomes. It plays a key role in pigmentation. MART-1 plays an important role in the expression, stability, trafficking and processing of Pmel17.
Melanoma-associated antigen recognized by T cells-1 (MART-1) has been shown to be essential for the functioning of glycoprotein-100 (gp100).

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Enhanced generation of specific tumor-reactive CTL in vitro by selected Melan-A/MART-1 immunodominant peptide analogues.
Valmori D, et al.
Journal of Immunology, 160(4), 1750-1758 (1998)
Gene expression profiling of human melanoma cell lines with distinct metastatic potential identifies new progression markers.
BERTUCCI F, et al.
Anticancer Research, 27(5A), 3441-3449 (2007)
MART-1 is required for the function of the melanosomal matrix protein PMEL17/GP100 and the maturation of melanosomes.
Hoashi T, et al.
The Journal of Biological Chemistry, 280(14), 14006-14016 (2005)
María Marcela Barrio et al.
PloS one, 7(7), e40311-e40311 (2012-07-07)
Dendritic cells (DC) can achieve cross-presentation of naturally-occurring tumor-associated antigens after phagocytosis and processing of dying tumor cells. They have been used in different clinical settings to vaccinate cancer patients. We have previously used gamma-irradiated MART-1 expressing melanoma cells as

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