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SAB4504208

Sigma-Aldrich

Anti-phospho-Smad2/3 (pThr8) antibody produced in rabbit

affinity isolated antibody

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Synonym(s):
Anti-HSPC193, Anti-HsT17436, Anti-JV15-2, Anti-LDS1C, Anti-LDS3, Anti-MADH3
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 48 kDa

species reactivity

rat, mouse, human

concentration

~1 mg/mL

technique(s)

ELISA: 1:10000
western blot: 1:500-1:1000

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pThr8)

Gene Information

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This Item
SAB4504207SAB4503781SAB4501808
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

Quality Level

100

Quality Level

-

Quality Level

-

Quality Level

-

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

UniProt accession no.

P84022

UniProt accession no.

Q15796

UniProt accession no.

P84022

UniProt accession no.

Q15796

technique(s)

ELISA: 1:10000, western blot: 1:500-1:1000

technique(s)

ELISA: 1:10000, western blot: 1:500-1:1000

technique(s)

ELISA: 1:40000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

ELISA: 1:1000, immunofluorescence: 1:100-1:500, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

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General description

SMAD3 (homologues of the Drosophila protein, mothers against decapentaplegic (Mad) and the Caenorhabditis elegans protein) is located on human chromosome 15q22. SMAD2 (SMAD family member 2) is also called as MADR2 (substrate of the TGFβ receptor). It is located on human chromosome 18q21. Smad2/3 belongs to the mothers against Dpp (MAD) related family of proteins. SMAD2 has two highly conserved amino and carboxyl-terminal domains (MH1 and MH2 domains) but has no known structural motifs.

Immunogen

The antiserum was produced against synthesized peptide derived from human Smad2/3 around the phosphorylation site of Thr8.

Immunogen Range: 1-50

Application

Anti-phospho-Smad3 (pSer425) antibody has been used in western blotting.

Biochem/physiol Actions

SMAD (homologues of the Drosophila protein, mothers against decapentaplegic (Mad) and the Caenorhabditis elegans protein) proteins play an important role in the intracellular signalling of transforming growth factor β (TGFβ). Reduction in the phosphorylation level of Smad3 helps autophagy to control the endothelial-mesenchymal transition. In human arterial smooth muscle cells, suppressing siRNA of SMAD3 improves the viability of cells. Mutations in SMAD2 (SMAD family member 2) results in arterial aneurysms and dissections. Upregulation of Smad2 blocks the multiplication of gingival epithelial cells. It plays an important role in TGF-β (transforming growth factor β)/activin signaling pathways. Smad2 positively and negatively controls TGFβ-dependent transcription with the help of forkhead DNA-binding protein FAST2.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Jiaqiang An et al.
Oncology reports, 38(5), 3001-3010 (2017-10-20)
In the past decades, altered Follistatin‑like 1 (FSTL1) expression has been documented in a variety of cancers, while its functional roles are poorly understood. Particularly in breast cancer, the expression of FSTL1 and its signaling pathway remain to be determined. In the
Smad2 and Smad3 Positively and Negatively Regulate TGF?-Dependent Transcription through the Forkhead DNA-Binding Protein FAST2
Labbe E, et al.
Molecular Cell (1998)
Frequency of Smad Gene Mutations in Human Cancers'
Riggins G J, et al.
Cancer Research (1997)
Peng Feng et al.
iScience, 26(12), 108484-108484 (2023-12-14)
Fibrosis disrupts tissue balance and links to severe illnesses, impairing organ function and, in some cases, even fatality. The interaction between M2 macrophages and fibroblasts is vital for tissue equilibrium. Transforming growth factor β1 (TGF-β1) released by M2 macrophages plays
Absence of chloride intracellular channel 4 (CLIC4) predisposes to acute kidney injury but has minimal impact on recovery
Edwards JC, et al.
BMC Nephrology (2014)

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