MilliporeSigma
All Photos(6)

Documents

SAB4504403

Sigma-Aldrich

Anti-phospho-FAK (pTyr397) antibody produced in rabbit

affinity isolated antibody

Sign Into View Organizational & Contract Pricing

NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 119 kDa

species reactivity

rat, mouse, human

concentration

~1 mg/mL

technique(s)

ELISA: 1:5000
western blot: 1:500-1:1000

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr397)

Gene Information

human ... PTK2(5747)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SAB4504181SAB5700348SAB4301455
conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

-

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

antigen 119 kDa

mol wt

antigen 119 kDa

mol wt

114, 119, 120, 39, 48, 63, 99

mol wt

125 kDa

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Protein tyrosine kinase 2 (PTK2), also known as FAK (focal adhesion kinase), is encoded by the gene mapped to human chromosome 8q24-qter. The encoded protein is localized to cell adhesion sites and extracellular matrix.

Immunogen

The antiserum was produced against synthesized peptide derived from human FAK around the phosphorylation site of Tyr397.

Immunogen Range: 364-413

Biochem/physiol Actions

Protein tyrosine kinase 2 (PTK2) is a non-receptor tyrosine kinase which is known to regulate cell migration, proliferation, cell survival and invasiveness. Increased expression of FAK (focal adhesion kinase) leads to metastatic breast tumors and lung cancer. Inhibition of FAK tyrosine kinase activity stops various cellular process necessary for tumor growth. Hence, FAK inhibitors are considered to be potential therapeutic methods for cancer treatment. FAK is one of the important components of integrin adhesion complexes (IACs)and it plays a vital role in maintaining IAC dynamics. Cell interactions with components of extracellular matrix and a number of agonists, such as neuropeptides, activate Ptk2. Ptk2, mediates cell signaling through its kinase dependent/independent functions. Upregulation of the gene is observed in hepatocellular carcinoma, which is accompanied with portal venous invasion and intrahepatic metastasis.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

F T Fiedorek et al.
Mammalian genome : official journal of the International Mammalian Genome Society, 6(2), 123-126 (1995-02-01)
Focal adhesion kinase (pp125FAK or FAK) is a cytoplasmic protein-tyrosine kinase stimulated in response to cell interactions with extracellular matrix components and by exposure to a variety of agonists, including neuropeptides. FAK lacks Src-homology SH2 and SH3 domains, is highly
Focal adhesion kinase inhibitors in combination with erlotinib demonstrate enhanced anti-tumor activity in non-small cell lung cancer.
Howe G A, et al.
PLoS ONE, 11(3), e0150567-e0150567 (2016)
MicroRNA-7 inhibits cell proliferation, migration and invasion in human non-small cell lung cancer cells by targeting FAK through ERK/MAPK signaling pathway.
Cao Q, et al.
Oncotarget, 7(47), 77468-77468 (2016)
T Konry et al.
Talanta, 75(2), 564-571 (2008-03-29)
We present herein an effective and versatile method to fabricate a micro-patterned structure of conductive polymer, poly(pyrrole-benzophenone), on Indium Tin Oxide (ITO) glass chips for the subsequent photo-immobilization of various bioreceptor, antigens. Such methodologies are based on photolithography of ITO
Iliet Payan et al.
International journal of oncology, 48(5), 2043-2054 (2016-03-18)
Focal adhesion kinase (FAK) is a non-receptor tyrosine kinase known to promote cell migration and invasiveness. Overexpression and increased activity of FAK are closely associated with metastatic breast tumors and are linked to poor prognosis. This study discovered an inverse

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service