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SAB4600299

Sigma-Aldrich

Anti-Mouse IgG (H+L), F(ab′)2 fragment, CF555 antibody produced in goat

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

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NACRES:
NA.46

biological source

goat

Quality Level

conjugate

CF 555 conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

concentration

~2 mg/mL

technique(s)

flow cytometry: 1-10 μg/mL
immunocytochemistry: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
indirect immunofluorescence: 1-10 μg/mL
western blot: suitable

fluorescence

λex 555 nm; λem 565 nm

storage temp.

−20°C

target post-translational modification

unmodified

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antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

goat

biological source

goat

biological source

goat

biological source

goat

conjugate

CF 555 conjugate

conjugate

CF 594 conjugate

conjugate

CF 594 conjugate

conjugate

CF 555 conjugate

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

rabbit

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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General description

CF555 F(ab′)2 fragment of goat anti-mouse IgG is affinity-purified F(ab′)2 fragment of goat anti-mouse IgG labeled with a orange-red fluorescent dye CF555, one of an outstanding series of CF dyes developed by Biotium. CF dyes are superior to both Alexa Fluor dyes and Cy dyes for antibody labeling by having combined advantages in brightness, photostability, and specificity.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. IgA molecules in the serum are mostly dimeric. IgA is found in saliva, tracheobronchial secretions, genitourinary secretions, milk and colostrum. IgM is present on the surface of B lymphocytes in its monomeric form.

Specificity

Binds with whole molecule mouse IgG and the light chains of other mouse immunoglobulins.

Immunogen

mouse IgG(H+L)

application

Anti-Mouse IgG (H+L), F(ab′)2 fragment, CF 555 antibody has been used in immunofluorescence.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG2a has cytotropic properties and IgG2 molecules have the ability to activate the complement system.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 0.2% BSA.

Preparation Note

Protect from light. The antibody solution should be gently mixed before use.

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
CF is a trademark of Biotium, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Swertisin ameliorates diabetes by triggering pancreatic progenitors for islet neogenesis in Streptozotocin treated BALB/c mice.
Abhay S, et al.
Biomedicine and Pharmacotherapy, 100, 221-225 (2018)
Nidheesh Dadheech et al.
Scientific reports, 12(1), 21496-21496 (2022-12-14)
Poly(ADP-ribose) polymerase-1 (PARP1), a fundamental DNA repair enzyme, is known to regulate β cell death, replication, and insulin secretion. PARP1 knockout (KO) mice are resistant to diabetes, while PARP1 overactivation contributes to β cell death. Additionally, PARP1 inhibition (PARPi) improves
Alma Nazlie Mohebiany et al.
Cell reports, 30(5), 1585-1597 (2020-02-06)
Tumor-necrosis-factor-alpha-induced protein 3 (TNFAIP3), or A20, is a ubiquitin-modifying protein and negative regulator of canonical nuclear factor κB (NF-κB) signaling. Several single-nucleotide polymorphisms in TNFAIP3 are associated with autoimmune diseases, suggesting a role in tissue inflammation. While the role of
Nidheesh Dadheech et al.
Stem cell research & therapy, 11(1), 327-327 (2020-08-01)
Despite the potential, bone marrow-derived mesenchymal stem cells (BMSCs) show limitations for beta (ß)-cell replacement therapy due to inefficient methods to deliver BMSCs into pancreatic lineage. In this study, we report TGF-ß family member protein, Activin-a potential to stimulate efficient
Specific IgG for cat allergens in patients with allergic conjunctivitis.
Miyama A, et al.
International Ophthalmology, 35(4), 575-586 (2015)

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