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SAB4700518

Sigma-Aldrich

Monoclonal Anti-CD71-FITC antibody produced in mouse

clone MEM-75, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Anti-TFRC, Anti-Transferrin Receptor
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MEM-75, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

flow cytometry: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... TFRC(7037)

Related Categories

General description

Transferrin receptor (TFRC, p90) is also known as cluster of differentiation 71 (CD71). The antibody MEM-75 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. In human chromosome, the gene TFRC is localized on 3q29.

Immunogen

NALM-6 human pre-B cell line

Application

The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 1e6 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.

Biochem/physiol Actions

Transferrin receptor (TFRC, CD71) facilitates the uptake of iron bound to transferrin (Tf) through clathrin-mediated endocytosis. TFRC interacts with iron-bound holo-transferrin at pH 7.4 and does not interact apo-transferrin. TFRC also binds with H-ferritin. Expression level of TFRC is regulated stringently by intracellular iron levels. The expression is higher in erythroblasts owing to haemoglobin synthesis. The expression is also higher in cancer cells, osteoclasts and activated lymphocytes, where iron requirement is high. Hypoxia and iron deficiency also increases TFRC expression. Recycling of Tf and TFRC is essential for the uptake of iron from the serum. Mutations in the internalization motif of TFRC leads to impaired endocytosis and consequently congenital/combined immunodeficiency. Improper palmitoylation of TFRC is connected to neurodegeneration with brain iron accumulation. Upregulation of TFRC is implicated in progression of pancreatic cancer, adult T-cell leukemia/lymphoma (ATLL), and cholangiocarcinoma.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Development of a complete human igg monoclonal antibody to transferrin receptor 1 targeted for adult T-cell leukaemia/lymphoma
Shimosaki S, et al.
Biochemical and Biophysical Research Communications, 485(1), 144-151 (2017)
A missense mutation in TFRC, encoding transferrin receptor 1, causes combined immunodeficiency
Jabara HF, et al.
Nature Genetics, 48(1), 74-74 (2016)
Upregulation of transferrin receptor-1 induces cholangiocarcinoma progression via induction of labile iron pool
Jamnongkan W, et al.
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine, 39(7), 1-10 (2017)
Impaired transferrin receptor palmitoylation and recycling in neurodegeneration with brain iron accumulation
Drecourt A, et al.
American Journal of Human Genetics, 102(2), 266-277 (2018)
NotI linking/jumping clones of human chromosome 3: mapping of the TFRC, RAB7 and HAUSP genes to regions rearranged in leukaemia and deleted in solid tumours
Kashuba VI, et al.
Febs Letters, 419(2-3), 181-185 (1997)

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