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SAE0095

Sigma-Aldrich

Agrin Human

Recombinant, cell culture tested, expressed in HEK 293 cells

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Synonym(s):
AGRN, Proteoglycan

recombinant

expressed in HEK 293 cells

assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

attachment assay, measured by the ability of the immobilized agrin to support the adhesion of PC12 cells

technique(s)

cell culture | mammalian: suitable

suitability

endotoxin tested

shipped in

ambient

storage temp.

−20°C

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This Item
I17001SAE0075E5546
vibrant-m

SAE0095

Agrin Human

vibrant-m

I17001

Interferon-γ human

vibrant-m

E5546

Erythropoietin (EPO) human

assay

≥95% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

shipped in

ambient

shipped in

-

shipped in

dry ice

shipped in

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

potency

attachment assay, measured by the ability of the immobilized agrin to support the adhesion of PC12 cells

potency

≤0.250 ng/mL In Viral Resistance Assay ED50

potency

-

potency

≤2.5 ng/mL ED50 (measured in a cell proliferation assay using TF-1 human cells)

General description

Agrin C-terminal human recombinant is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 87 kDa (amino acids Ala1260-Pro2045, with an N-terminal His tag). The DTT-reduced protein migrates as a ∼100 kDa polypeptide on SDS-PAGE due to glycosylation. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher bioactivity of the protein.

Biochem/physiol Actions

Agrin is a large extracellular heparan sulfate proteoglycan that is involved in the formation of the neuromuscular junction.
Agrin triggers the aggregation of acetylcholine receptors via the muscle-specific kinase (MuSK) and the low-density lipoprotein receptor-related protein 4 (Lrp4) receptor complex.
Agrin is required for the full regenerative capacity of neonatal mouse hearts.
Recombinant agrin has been shown in vitro to promote cardiomyocyte proliferation, as studied in mouse-induced and human-induced pluripotent stem cells. Recombinant agrin has been shown in vivo to promote cardiac regeneration, after induction of myocardial infarction in adult mice.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2


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Xiu-Qing Fu et al.
Cell discovery, 6, 9-9 (2020-03-07)
During the development of mammalian neuromuscular junction (NMJ), the original supernumerary axon inputs are gradually eliminated, finally leaving each muscle fiber innervated by a single axon terminal. However, the molecular cues that mediate the elimination of redundant axon inputs remain

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