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SCJ4600042

Sigma-Aldrich

CF 640R hydrazide

suitable for fluorescence

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NACRES:
NA.41

form

solid

Quality Level

ε (extinction coefficient)

105000 at 642 nm

absorbance ratio

A280/642 nm 0.37

fluorescence

λex 633, 635 or 640 nm (Flow cytometry laser line)
λex 633, 635 or 640 nm (Microscopy laser line)
λex 642 nm; λem 662 nm

suitability

suitable for fluorescence

storage temp.

−20°C

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SCJ4600043SCJ4600040SCJ4600041
CF™ 640R hydrazide suitable for fluorescence

SCJ4600042

CF 640R hydrazide

Sigma-Aldrich

SCJ4600043

CF 640R maleimide

Sigma-Aldrich

SCJ4600040

CF 640R amine

Sigma-Aldrich

SCJ4600041

CF 640R aminooxy

fluorescence

λex 633, 635 or 640 nm (Flow cytometry laser line), λex 642 nm; λem 662 nm, λex 633, 635 or 640 nm (Microscopy laser line)

fluorescence

λex 633, 635 or 640 nm (Flow cytometry laser line), λex 633, 635 or 640 nm (Microscopy laser line), λex 642 nm; λem 662 nm

fluorescence

λex 633, 635 or 640 nm (Flow cytometry laser line), λex 633, 635 or 640 nm (Microscopy laser line), λex 642 nm; λem 662 nm

fluorescence

λex 633, 635 or 640 nm (Flow cytometry laser line), λex 633, 635 or 640 nm (Microscopy laser line), λex 642 nm; λem 662 nm

form

solid

form

solid

form

solid

form

solid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

ε (extinction coefficient)

105000 at 642 nm

ε (extinction coefficient)

105000 at 642 nm

ε (extinction coefficient)

105000 at 642 nm

ε (extinction coefficient)

105000 at 642 nm

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

CF640R is a rhodamine-based far-red fluorescent dye with excitation and emission maxima very similar to those of Cy®5 and Alexa Fluor 647. CF640R is much brighter than Cy®5 and at least as bright as Alexa Fluor 647. A major advantage of CF640R over Cy® 5 and Alexa Fluor 647 is its exceptional photostability. Cy®5 and Alexa Fluor 647 are cyanine-based dyes and, like other cyanine dyes in general, have intrinsically poor photostability. CF640R is also far brighter and more photostable than Atto 647N, another spectrally similar dye frequently used in single-molecule imaging. The combination of excellent brightness and photostability makes CF640R ideal for confocal microscopy, single- molecule imaging and other demanding applications based on fluorescence detection.

CF dye hydrazides can be used as a fixable polar tracers for visualizing cell morphology,or for gap junction studies. The dyes can be introduced into cells by microinjection. For conjugation to aldehyde or ketone groups (such as carbohydrate molecules after peroxidation with periodate) we recommend using the more reactive CF aminooxy dye forms.

Features and Benefits

1. Has the best photostability among dyes with Cy®5-like spectra. 2. Yields highly fluorescent protien conjugates. 3. Very water-soluble and pH-insensitive.

Preparation Note

Protect from light

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
CF is a trademark of Biotium, Inc.
Cy is a registered trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Hendrik Kohlhof et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 38(6), 1359-1364 (2019-12-24)
Periprosthetic joint infection is among the most common and severe complications in total joint arthroplasty. Today, a combination of different methods is used for diagnosis because no single method with sufficient sensitivity and specificity is available. In this study, we

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