SMB00288

Peptidoglycan from Bacillus subtilis

≤1.00 EU/mg Endotoxin

• Synonym(s): PGN
• MDL number: MFCD00212486
• UNSPSC Code: 12352201
• NACRES: NA.25

Properties

• biological source: Bacillus subtilis
• Quality Level: 200
• form: lyophilized powder
• impurities: ≤1.00 EU/mg Endotoxin
• solubility: water: 9.80-10.20 mg/mL, hazy to turbid
• storage temp.: −20°C

Description

General description

Peptidoglycan.is a biopolymer that is a major component of the cell walls of Gram-positive bacteria. Peptidoglycan consists of a carbohydrate backbone of alternating units of N-acetyl glucosamine and N-acetyl muramic acid and is modified with both D- and L-amino acids. N-Acetyl muramic acid residues are cross-linked with oligopeptides. The terminal peptide is D-alanine although other amino acids are present as D-isomers.

Application

Peptidoglycan may be used for the activity estimation of lytic enzymes (e.g. lyticase). It is recommended to use a peptidoglycan concentration of 0.15-3 μg/ml in water or buffer and measuring at 450 nm. The peptidoglycan is not soluble but produces a suspension in water.
Peptidoglycan activates the Toll-like receptor 2 (TLR2), present in mammalian cells for the stimulation of lymphocytes. It may also function as an antagonist of Poly (I:C).

Other Notes

To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable