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SRP0128

Sigma-Aldrich

DNMT3a Active human

recombinant, expressed in baculovirus infected insect cells

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Synonym(s):
DNA (cytosine-5-)-methyltransferase 3A, DNA MTase HsaIIIA, DNMT3A2, M.HsaIIIA
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected insect cells

form

aqueous solution

mol wt

128 kDa

packaging

pkg of 10 μg

concentration

>0.02 mg/mL

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... DNMT3A(1788)

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vibrant-m

SRP0128

DNMT3a Active human

vibrant-m

SRP0166

DNMT3B/DNMT3L Active human

vibrant-m

SRP0126

DNMT1 Active human

Gene Information

human ... DNMT3A(1788)

Gene Information

human ... DNMT3A(1788)

Gene Information

human ... DNMT3B(1789)

Gene Information

human ... DNMT1(1786)

recombinant

expressed in baculovirus infected insect cells

recombinant

-

recombinant

expressed in baculovirus infected insect cells

recombinant

expressed in baculovirus infected insect cells

storage temp.

−70°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−70°C

concentration

>0.02 mg/mL

concentration

2.24 mg/ml

concentration

>0.02 mg/mL

concentration

0.12 mg/mL

mol wt

128 kDa

mol wt

101, 16, 81

mol wt

27 kDa (DNMT3L), 59 kDa (DNMT3B)

mol wt

211 kDa

General description

DNMT3A (DNA methyltransferase 3α) is one of three (DNMT1, DNMT3a and DNMT3b) active forms of DNA methyltransferases. This gene is localized to human chromosome 2p23. DNMT3A is one of the three members of the DNMT3A family, of which DNMT3A and DNMT3B are catalytically active and DNMT3L is an inactive paralogue, which has regulatory actions in germ cells.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

DNA methyltransferases are responsible for the addition of a methyl residue to the cytosine group of CpG dinucleotides. DNMT3A (DNA methyltransferase 3α) participates in the gene expression suppression by interacting with histone modifiers, such as polycomb-group (PcG). The interaction between these two proteins is thought to be implicated in cancer pathogenesis. During embryogenesis this enzyme is responsible for the formation of genomic methylation patterns and germ line development. Polymorphisms in this gene might serve as biomarkers to determine the prognosis of gastric cancer patients. Mutations in this gene are linked with Tatton-Brown-Rahman syndrome, an overgrowth syndrome characterized by unique facial appearance, intellectual disability, and increased height.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

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Tatton-Brown-Rahman syndrome due to 2p23 microdeletion.
Okamoto N et al
American Journal of Medical Genetics. Part A, 170A(5), 1339-1342 (2016)
DNMT3A mutations in acute myeloid leukemia.
Ley TJ et al
The New England Journal of Medicine, 363(25), 2424-2433 (2010)
Structure of Dnmt3a bound to Dnmt3L suggests a model for de novo DNA methylation.
Jia D et al
Nature, 449(7159), 248-251 (2007)
DNMT3A R882 mutants interact with polycomb proteins to block haematopoietic stem and leukaemic cell differentiation.
Koya J et al
Nature Communications, 7, 10924-10924 (2016)
DNA methyltransferase 3a rs1550117 genetic polymorphism predicts poor survival in gastric cancer patients.
Wang C et al
International Journal of Clinical and Experimental Pathology, 8(11), 14864-14874 (2015)

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