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SRP0212

Sigma-Aldrich

PTP1B (1-321)/PTPN1 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

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Synonym(s):
PTPN1, Protein tyrosine phosphatase, non-receptor type 1
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

63.3 kDa

packaging

pkg of 20 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

0.84 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PTPN1(5770)

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Sigma-Aldrich

SRP0212

PTP1B (1-321)/PTPN1 Active human

Sigma-Aldrich

SRP0215

PTP1B full length Active human

Sigma-Aldrich

SRP0211

PTP-D2/PTPN14 Active human

biological source

human

biological source

human

biological source

mouse

biological source

human

assay

≥90% (SDS-PAGE)

assay

≥55% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

form

aqueous solution

form

aqueous solution

form

aqueous solution

form

aqueous solution

concentration

0.84 mg/mL

concentration

1.65 mg/mL

concentration

>0.02 mg/mL

concentration

>0.02 mg/mL

General description

Protein tyrosine phosphatase, catalytic domain, (amino acids 1-321), (Gene bank accession no. M31724) with N-terminal GST tag, MW = 63.3 kDa, expressed in an E. coli expression system.
Protein tyrosine phosphatase, non-receptor type 1 (PTPN1), which is also known as protein-tyrosine phosphatase 1B (PTP1B), is a tyrosine phosphatase. It is localized at the cytoplasmic side of the endoplasmic reticulum. The gene encoding PTPN1 is localized on human chromosome 20q13.13.

application

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

Biochem/physiol Actions

Protein tyrosine phosphatase, non-receptor type 1 (PTPN1) or protein-tyrosine phosphatase 1B (PTP1B) dephosphorylates the epidermal growth factor receptor. It acts as a modulator of insulin-like growth factor receptor (IGFR) and MET-mediated signaling responses. It may be useful as a drug target in type 2 diabetes and obesity. The protein has been shown to enhance tumor progression in various cancers such as breast, colon and prostate. It acts a tumor suppressor in other cancers (lymphomas and esophageal cancers).

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 Mm Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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