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SRP3070

Sigma-Aldrich

IGF-II human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

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Synonym(s):
Insulin-like Growth Factor-II
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤2.0 ng/mL ED50

mol wt

7.5 kDa

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

off-white to yellow

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IGF2(3481)

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IGF-II human

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IGF-Binding Protein-3 human

recombinant

expressed in E. coli

recombinant

expressed in (BTI-Tn-5B1-4) High-5 Insect Cells

recombinant

expressed in E. coli

recombinant

expressed in E. coli

biological source

human

biological source

human

biological source

human

biological source

human

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

mol wt

7.5 kDa

mol wt

31.5 kDa

mol wt

25.4 kDa

mol wt

28.8 kDa

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Insulin-like growth factors (IGFs) are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but, IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and three intra-molecular disulfide bonds.

Application

IL-4 from mouse has been used to activate B-cells.

Biochem/physiol Actions

The insulin-like growth factors (IGFs) are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro.

Sequence

AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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PAPP-A-Specific IGFBP-4 Proteolysis in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
Adasheva, et al.
International Journal of Molecular Sciences, 24 (2023)
Myc Regulates Chromatin Decompaction and Nuclear Architecture during B Cell Activation
Kyong-RimKieffer-Kwon
Molecular Cell, 67(4), 566-578 (2017)
Role of IGF-I Signaling in Muscle Bone Interactions
Daniel D, et.al
Bone, 80, 79-88 (2015)
Role of IGF-I Signaling in Muscle Bone Interactions
Daniel D Bikle
Bone, 80, 79-88 (2015)
Type 1 Insulin-like Growth Factor Receptor (IGF-IR) Signaling Inhibits Apoptosis Signal-regulating Kinase 1 (ASK1)*
Veronica Galvan
The Journal of Biological Chemistry, 13325-13332 (2003)

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