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SRP5005

Sigma-Aldrich

CDC25A, active, GST tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

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Synonym(s):
CDC25A2
NACRES:
NA.32

recombinant

expressed in baculovirus infected Sf9 cells

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

26-36 nmol/min·mg

mol wt

~94 kDa

NCBI accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CDC25A(993)

General description

CDC25A (also known as cell division cycle 25 homolog A) is a member of the CDC25 family of phosphatases that are required for progression from G1 to the S phase of the cell cycle. CDC25A can activate the cyclin-dependent kinase CDC2 (also known as CDK1) by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A overexpression is detected in human cancers and this may contribute to the tumorigenesis process. CDC25A is degraded by moderate heat shock and this degradation is protected by HSP90.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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