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SRP5251

Sigma-Aldrich

PKC-α, active, GST tagged from Xanopus sp.

PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

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Synonym(s):
MGC82897, PRKCA
NACRES:
NA.32

biological source

Xenopus sp.

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO®

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

186-290 nmol/min·mg

mol wt

~103 kDa

NCBI accession no.

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

Xenopus laevis ... prkca(447031)

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This Item
SRP5252SRP5332K4143
PKC-δ, active, GST tagged human PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

SRP5252

PKC-δ, active, GST tagged human

GLK, active, GST tagged human PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

SRP5332

GLK, active, GST tagged human

Sigma-Aldrich

K4143

PKCη, active, GST tagged human

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

biological source

Xenopus sp.

biological source

human

biological source

human

biological source

-

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

mol wt

~103 kDa

mol wt

~104 kDa

mol wt

~70 kDa

mol wt

~73 kDa

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

Protein kinase C-α (PKC-α) is a member of the PKC family of serine- and threonine-specific protein kinases that can be activated by calcium and the secondary messenger diacylglycerol. The protein is expressed in the dorsal ectoderm of Xenopus.

Biochem/physiol Actions

Protein kinase C-α (PKC-α) has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint and cell volume control. It has been identified as a fundamental regulator of cardiac contractility and Ca2+ handling in myocytes.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Preparation Note

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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PKC-alpha regulates cardiac contractility and propensity toward heart failure.
Braz JC
Nature Medicine, 10(3), 248-254 (2004)
aPKC phosphorylates p27Xic1, providing a mechanistic link between apicobasal polarity and cell-cycle control.
Sabherwal N
Developmental Cell, 31(5), 559-571 (2014)
Protein kinase C isozymes have distinct roles in neural induction and competence in Xenopus.
Otte AP and Moon RT
Cell, 68(6), 1021-1029 (1992)
Yi Liu et al.
The Biochemical journal, 478(10), 1999-2017 (2021-05-08)
Human hepatic tryptophan 2,3-dioxygenase (hTDO) is a homotetrameric hemoprotein. It is one of the most rapidly degraded liver proteins with a half-life (t1/2) of ∼2.3 h, relative to an average t1/2 of ∼2-3 days for total liver protein. The molecular mechanism

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