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SRP5319

Sigma-Aldrich

PI3K (p110-α/p55-γ), active, His tagged human

PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

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Synonym(s):
p110-alpha, p55-gamma
NACRES:
NA.32

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO®

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

11.2-16.8 nmol/min·mg

mol wt

~111 kDa (p110α)
~61 kDa (p85γ)

NCBI accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PIK3CA(5290)

General description

PI3K (p110alpha/p55gamma) or Phosphatidylinositol 3-kinase is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position which serve as second messengers in growth signaling pathways. PI3K comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in the PI3K has been associated with insulin resistance and plays an important role in glucose homeostasis in vivo. PI3K also plays an essential role in the development and induction of mast cells in normal and pathogenic immune responses.

Physical form

Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol.

Preparation Note

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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