MilliporeSigma
All Photos(1)

Documents

SRP6371

Sigma-Aldrich

Aldose Reductase human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

Sign Into View Organizational & Contract Pricing

Synonym(s):
ADR, AKR1B1, ALDR1, ALR2, AR, Aldo-keto reductase family1, member B1
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE)

form

liquid

mol wt

35.8 kDa (316 aa, 1-316 aa)

packaging

pkg of 100 μg

concentration

1 mg/mL

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... AKR1B1(231)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SAB1410405SRP6090SAB2100091
vibrant-m

SRP6371

Aldose Reductase human

vibrant-m

SRP6090

AKR1B10 human

recombinant

expressed in E. coli

recombinant

-

recombinant

expressed in E. coli

recombinant

-

concentration

1 mg/mL

concentration

-

concentration

-

concentration

0.5 mg - 1 mg/mL

biological source

human

biological source

rabbit

biological source

human

biological source

rabbit

mol wt

35.8 kDa (316 aa, 1-316 aa)

mol wt

antigen 34.76 kDa

mol wt

36 kDa

mol wt

36 kDa

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

General description

Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily. Recombinant aldose reductase (AKR1B) protein was expressed in Escherichia coli and purified by using conventional chromatography techniques. It is expressed in eye and kidney, but predominant in placenta. The AKR1B1 gene is mapped to human chromosome 7q33.

Biochem/physiol Actions

Aldose reductase (AKR1B1) catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. AKR1B1 is also involved in cytokine-mediated generation of prostaglandin F2α (PGF2α) in preadipocytes. It participates in diabetes-associated mitochondrial disorder and damage via the activation of p53. AKR1B1 is associated with drug resistance mechanism in cancer-derived cell lines. It is also known to mediate cellular processes linked to carcinogenesis such as EMT (epithelial–mesenchymal transition), inflammation and angiogenesis. Aldose reductase mediates glucose metabolism in placenta and osmotic control in eye and kidney.

Physical form

1 mg/mL in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT and 10% glycerol.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

The human aldose reductase AKR1B1 qualifies as the primary prostaglandin F synthase in the endometrium.
Bresson E, et al.
The Journal of Clinical Endocrinology and Metabolism, 96, 210-219 (2011)
Prostaglandin (PG) F2 alpha synthesis in human subcutaneous and omental adipose tissue: modulation by inflammatory cytokines and role of the human aldose reductase AKR1B1.
Michaud A, et al.
PLoS ONE, 9, e90861-e90861 (2014)
Is rs759853 polymorphism in promoter of aldose reductase gene a risk factor for diabetic nephropathy? A meta-analysis.
Cui W, et al.
European Journal of Medical Research, 20, 14-14 (2015)
The crystal structure of the aldose reductase.NADPH binary complex.
Borhani DW, et al.
The Journal of Biological Chemistry, 267, 24841-24847 (1992)
Expression of the Aldo-Ketoreductases AKR1B1 and AKR1B10 in Human Cancers.
Laffin B and Petrash JM
Frontiers in Pharmacology, 3, 104-104 (2012)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service