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SRP6410

Sigma-Aldrich

ApoA-1 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

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Synonym(s):
Apolipoprotein A-I, MGC117399
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

tag

6-His tagged (C-terminus)

Assay

≥95% (SDS-PAGE)

form

lyophilized

mol wt

calculated mol wt 29 kDa
observed mol wt 26-30 kDa (DTT-reduced. Asp 25 is the predicted N-terminal.)

packaging

pkg of 10 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... ApoA1(335)

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SRE0012MSST0001SRP6464
ApoA-1 human recombinant, expressed in HEK 293 cells, &#8805;95% (SDS-PAGE)

Sigma-Aldrich

SRP6410

ApoA-1 human

ApoJ/Clusterin human recombinant, expressed in HEK 293 cells, &#8805;92% (SDS-PAGE)

Sigma-Aldrich

SRP6464

ApoJ/Clusterin human

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

assay

≥95% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

≥92% (SDS-PAGE)

form

lyophilized

form

lyophilized powder

form

lyophilized powder

form

lyophilized

mol wt

calculated mol wt 29 kDa, observed mol wt 26-30 kDa (DTT-reduced. Asp 25 is the predicted N-terminal.)

mol wt

-

mol wt

-

mol wt

calculated mol wt 50.9 kDa (Asp23-Glu449, α chain 24.5kDa +β chain 27_kDa.The recombinant rh CLUS / Clusterin (Asp23-Glu 449) was cleaved into α chain and β chain, which form a heterodimer linked by disulfide bonds.), observed mol wt 34-37 kDa by SDS-PAGE (DTT reduced. corresponding to the cleaved β chain, andβ chain respectively.)

packaging

pkg of 10 μg

packaging

-

packaging

vial of ≥10 μg (Lot-specific vial content given on certificate of analysis)

packaging

pkg of 10 μg, pkg of 50 μg

General description

ApoA1 is also known as apolipoprotein A-I, ApoA-I , and is the major protein component of high density lipoprotein (HDL) in plasma. It has a specific role in lipid metabolism. Chylomicrons secreted from the intestinal enterocyte also contain ApoA1 but it is quickly transferred to HDL in the bloodstream. The protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I was also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. In addition, it has been shown that ApoA1 is implicated in the anti-endotoxin function of HDL via interaction with lipopolysaccharide or endotoxin.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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