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U4754

Sigma-Aldrich

Anti-Lectin, Ulex europaeus-I antibody produced in rabbit

whole antiserum, delipidized

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Synonym(s):
Anti-Gorse or Furze Lectin, Anti-UEA-I
MDL number:
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

15 mM sodium azide

species reactivity

Ulex europaeus

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
indirect immunofluorescence: 1:500 using human tissue

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
N3893V1514S1445
antibody form

whole antiserum

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

form

liquid

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect immunofluorescence: 1:500 using human tissue, immunohistochemistry (frozen sections): suitable

technique(s)

immunohistochemistry (frozen sections): 1:100 using acetone-fixed, frozen tissue sections of mouse heart, microarray: suitable, western blot: 1:10,000 using bovine lung endothelial cell extract

technique(s)

immunohistochemistry (frozen sections): 1:100 using acetone-fixed sections of human tonsil, western blot: 1:500 using whole extract of human umbilical vein endothelial cells (HUVEC).

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 8 μg/mL using human pancreas, Islets of Langerhans

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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General description

The antibody may be used for studies of the vascular structures. It reacts specifically with UEA-I bound to human endothelial cells of normal and neoplastic blood and lymphatic vessels. Its uniform reaction with vessels enables differentation between vessels and artificial slits in the tissue.

Immunogen

Ulex europaeus from gorse seeds..

Application

Anti-Lectin, Ulex europaeus-I antibody produced in rabbit has been used in double immunostaining of muscle capillary sections.

Biochem/physiol Actions

Lectin isolated from Ulex europaeus I is specific for α-L-fucose-containing glycocompounds. Due to this specificity it serves as a marker for vascular endothelial cells and tumors derived from them.

Target description

Lectins are highly specific carbohydrate binding proteins useful in carbohydrate and cell membrane studies, as well as cell agglutination and typing techniques.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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American journal of clinical pathology, 79(1), 32-36 (1983-01-01)
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Ulex europaeus I agglutinin, a lectin specific for some alpha-L-fucose-containing glycocompounds, was used in fluorescence microscopy to stain cryostat sections of human tissues. The endothelium of vessels of all sizes was stained ubiquitously in all tissues studied as judged by
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